Sequences from confirmation amplicon


Confirmation sequences for line 300D09 and hit At3g60510 are shown.
Line ID 300D09
Locus At3g60510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22358084
Primers used for PCR 17kh - 8474, show primer details
   
Sequence Name 76-K113669-0022-300-D09C-17kh
GenBank Accession LN513435 [GenBank]
Sequencing primer gene specific primer, 17kh: 5'-AATTAGTCGGACAGCTGGATTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K113669-0022-300-D09C-17kh
GCTCATGGATATTGCAGATTTTTGCTACCCCTGAAACAATCATCGGTTTTCATCCTGATG
CTGGTGCTTCTTTTAACCTCTCCCACTTACCTGGTCGCTTAGGTATGCTAGTGATATGTT
TTGAACTGCTTAAATTCTCTCTAGGTTTCTCATAATCTCATTTGACTGTGTTATTAACTG
CTGTAAAGCAATTTTTATTTGGACAATTACCATAGAAATCGCTGATCCAGCAGCAAAGTT
AAATATCAAATTCTGCTGTTTCATAGTATACATTAGCTCCCTCAGATGGACGATTTAATA
GAGTCTATGCAGGGGAGTATCTGGGTCTGACTGGGTTAAAGCTCAGTGGGGCAGAAATGT
TGGCATGTGGCCTTGCTACACATTATATTAGAAGTGAGGTAATTTAGATTATATAATTTG
AGATCTTTCTGTTTCCATAAAACTTATCTTACCTGCTAAATAGTATAATTTCTTGTCCTA
CAACAGGAGGTTCCTGTAATGGAAGAACAGCTCAAAAATGTCCGGGAAAAATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTT
   
Sequence Name 45-K113666-0022-300-D09C-8409
GenBank Accession LN505599 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K113666-0022-300-D09C-8409
TTTTTCCGGACATTTTTGAGCTGTTCTTCCATTACAGGAACCTCCTGTTGTAGGACAAGA
AATTATACTATTTAGCAGGTAAGATAAGTTTTATGGAAACAGAAAGATCTCAAATTATAT
AATCTAAATTACCTCACTTCTAATATAATGTGTAGCAAGGCCACATGCCAACATTTCTGC
CCCACTGAGCTTTAACCCAGTCAGACCCAGATACTCCCCTGCATAGACTCTATTAAATCG
TCCATCTGAGGGAGCTAATGTATACTATGAAACAGCAGAATTTGATATTTAACTTTGCTG
CTGGATCAGCGATTTCTATGGTAATTGTCCAAATAAAAATTGCTTTACAGCAGTTAATAA
CACAGTCAAATGAGATTATGAGAAACCTAGAGAGAATTTAAGCAGTTCAAAACATATCAC
TAGCATACCTAAGCGACCAGGTAAGTGGGAGAGGTTAAAAGAAGCACCAGCATCAGGATG
AAAACCGATGATTGTTTCAGGGGTAGCAAAAATCTGCAATATCCATGAGCTAGTTACTGC
ATCCTCCAAGAAGTTGGACATCCGAACTAAACTAGAATCCAGCTGTCCAACTAA

Last Updated on 06.12.2018 16:11