Sequences from confirmation amplicon


Confirmation sequences for line 300F01 and hit At5g49310 are shown.
Line ID 300F01
Locus At5g49310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19992275
Primers used for PCR CK54 - 8474, show primer details
   
Sequence Name 29-K018475-022-300-F01C-CK54
GenBank Accession LN499085 [GenBank]
Sequencing primer gene specific primer, CK54: 5'-GGAATCCACATTGTTTTGTCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K018475-022-300-F01C-CK54
CATCATATTATGTGCATGCTTTTGGTTCAATATGACAGCTATTTCTTTACATCAAAATCT
AATATCAGATTATGTGGGATCTTAACGAGAAAAATCAAACTGGAATTCCGGATGAGAACA
ACCATCACCACCTTCATCTTCCTCTTCAACTAACCTTCCATCTTCCTCTACAAGCCCGTA
TGTTTGCAGAATCTTCACAGCCTTCTCGTAGATCTCATTGTTGCCATGGTGCTGCAAGTT
CTCAATCTTTTCTAGCCCTTCTGCATCTTCAATCATTTGAGAGTAACAGTTCACGTCTCT
TAGGTTCTTGTCCACCTCTCCAGCAATCAAAATCATTTCCAATGGCTAAACCAGGATCAA
TTGTAAATGGCTTCTTGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATT
TATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA
CATTCAC
   
Sequence Name 30-K018475-022-300-F01C-8409
GenBank Accession LN499521 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K018475-022-300-F01C-8409
CATGTAGATTTCCCGGACAAGAAGCCATTTACAATTGATCCTGGTTTAGCCATTGGAAAT
GATTTTGATTGCTGGAGAGGTGGACAAGAACCTAAGAGACGTGAACTGTTACTCTCAAAT
GATTGAAGATGCAGAAGGGCTAGAAAAGATTGAGAACTTGCAGCACCATGGCAACAATGA
GATCTACGAGAAGGCTGTGAAGATTCTGCAAACATACGGGCTTGTAGAGGAAGATGGAAG
GTTAGTTGAAGAGGAAGATGAAGGTGGTGATGGTTGTTCTCATCCGGAATTCCAGTTTGA
TTTTTCTCGTTAAGATCCCACATAATCTGATATTAGATTTTGATGTAAAGAAATAGCTGT
CATATTGAACCAAAAGCATGCACATAATATGATGGTCCTAAAAGACAAAACAATGGNGAT
TC

Last Updated on 06.12.2018 16:11