SimpleSearch - Line and FST details


Line specific information

 
Line ID 300F01
Vector Used pAC161
Line Availability available as T3 set from NASC (N428765)
Segregation Analysis 100:4:4
Confirmed for Hit At5g49310
Parent of DUPLO pair none
Parent of pair(s) 4423, 7323, 90591, 90603, 90605, 90606

Gene hit F15H11

 
Sequence (A. th genome BLAST matches underlined) 
>06-K015599-022-300-F01-8409
TTTGATCCATGTAGATTTCCCGGACATGAAAGCATTTACAATTGAATACTGGCCTGCCCA
TTGATAACGATAAATGACTGTTGGGCACGAGCGCCTAACCCTATATACCCACTACTGCTA
CTCTCATTTACTAGAGCATGAGAAGAGTCTATGGGACCCCAAATTTGATCACAATAGTAC
TAATTAAGAATAATAATATAAACTGTTTACTTTTAAAGCGTTATTTGCTCATGTGAAACT
GGAACAAGGGAATATTCAAAATCAAAACCACACTTTTCTTCGCTAAAAAAAATCTACACA
ATAATCAACTAAGAGTTACCCGAACCTAATCGAAGAACACAAAAAAGAAAAAAAAAGGCG
GGGGAAAAAAAAAAAAGGGCCAAAAAAAAAAAAGGGGGTTTTCTTTTCCAATTTTTTATT
TAATTTTTTTTTGGCCCGGGCCCCCCGGGTTGGCCCCCCCTTTATGGGCGCCCTTTGGGC
CCCCCAAAAAATCCCCCCATTTTTTTTTTTAAAAAAATTGGGGAAAAAAAGGGGGTTTTG
GGGTGGGAAGGGGGGACAAAAAATTTCTAAAAAAATGCCCCCCCCTGGGG
GenBank Accession AL946994 [GenBank]
Graphic View Graphic view of sequence AL946994 of line 300F01
Predicted Position of Insertion Chr1:26746518 - go to primer design
BLAST e Value 1e-81
Hit Clone Code (BAC ID) F15H11
Insertion Classification Genome Hit
Confirmation Status unknown

Gene hit At5g49310

 
Sequence (A. th genome BLAST matches underlined) 
>06-K015600-022-300-F01-8409
NTGATCCATGTAGAATTTCCCGGACATGAAGCACTTTACAATTGATCCTGGTTTAGCCAT
TGGAAATGATTTTGATTGCTGGAGAGGTGGACAAGAACCTAAGAGACGTGAACTGTTACT
CTCAAATGATTGAAGATGCAGAAGGGCTATGGGATCCTCCCTATAGTGAGAACGAA
GenBank Accession CR356299 [GenBank]
Graphic View Graphic view of gene At5g49310
Predicted Position of Insertion Chr5:19992277 - go to primer design
BLAST e Value 6e-43
Hit Clone Code (BAC ID) K21P3
Hit Gene Code At5g49310 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation importin alpha isoform 5
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details


Last Updated on 10.06.2021 13:37