Sequences from confirmation amplicon


Confirmation sequences for line 302A10 and hit At5g56940 are shown.
Line ID 302A10
Locus At5g56940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23032267
Primers used for PCR BV09 - 8474, show primer details
   
Sequence Name 15-K105564-0022-302-A10C-BV09
GenBank Accession LN492970 [GenBank]
Sequencing primer gene specific primer, BV09: 5'-TATAACAAATGAAACTTCTCCGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K105564-0022-302-A10C-BV09
TTCTGGAATTGTTTCTTATTAGTGTAAGATGAAGAACCTGTTGATTATCTATTCGTATAC
ACACTTACTTACATTCTTAATTGTTCACCATATACAACCTCTTTTGCATAATTGTATGGT
CATGTAGCTTTAGAATCTTGTCATGCTCTTTATCATCATCTAAAATTAAGATTATAATGT
CCGGATATTAGAAGAACACATGTCAAGACTACTTAAAAGTTAGATCCTGGTCTGTCTTTC
TTTTGTTCTGAATGACTAGTTTCAACAGAGGATGCATATAGTTTGATTAATGGACATTTT
GTTTGATTCTCTTGTTCTGTACAGGTACTGGTTATCTGTTGGTGCTCAGCCATCAGACCC
GGTTCAACGTCTCCTATTCAGATCCGGTTTACTTCCTCCTCCTCCAATGGTGGCTATGGG
ACGTAAAGGTGGAGCACGAGACACACGCCCAGTTGATCCGATGACTGGTCGCTATGTGGA
TGCAGAGAATAAAACAGTTAATGCCAATGATAACCAGCCTAAGGAAGAGGATACAGAAGC
CAAGAGTGCATGATTCATTAGCCTTCTGTCATCGTAGCTTTTCAAGTTCACTTTGTTGTC
GATTATATTGTGTAGTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 63-K105565w-0022-302-A10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K105565w-0022-302-A10C-8409
TTACATTGAATATATCCTGCACTACACAATATAATCGACAACAAAGTGAACTTGAAAAGC
TACGATGACAGAAGGCTAATGAATCATGCACTCTTGGCTTCTGTATCCTCTTCCTTAGGC
TGGTTATCATTGGCATTAACTGTTTTATTCTCTGCATCCACATAGCGACCAGTCATCGGA
TCAACTGGGCGTGTGTCTCGTGCTCCACCTTTACGTCCCATAGCCACCATTGGAGGAGGA
GGAAGTAAACCGGATCTGAATAGGAGACGTTGAACCGGGTCTGATGGCTGAGCACCAACA
GATAACCAGTACCTGTACAGAACAAGAGAATCAAACAAAATGTCCATTAATCAAACTATA
TGCATCCTCTGTTGAAACTAGTCATTCAGAACAAAAGAAAGACAGACCAGGATCTAACTT
TTAAGTAGTCTTGACATGTGTTCTTCTAATATCCGGACATTATAATCTTAATTTTAGATG
ATGATAAAGAGCATGACAAGATTCTAAAGCTACATGACCATACAATTATGCAAAAGAGGT
TGTATATGGTGAACAATTAAGAATGTAAGTAAGTGTGTATACGAATAGATAATCAACAGG
TTCTTCATCTTACACTAATAAGAAACAATTCCAGAACTCAGAGCAGAAGTTCCATAGAAA
GACTCAAGACTGGCGGAGAA
Primers used for PCR AR15 - 8474, show primer details
   
Sequence Name 31-K118837-0022-302-A10C-AR15
GenBank Accession LN500355 [GenBank]
Sequencing primer gene specific primer, AR15: 5'-GTTTGAAAAGAGGTGGAGAGATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K118837-0022-302-A10C-AR15
CGACTAACGCAAAACGTGGCTTCCTCCTTTATAAAATTTAAACCTAACAACTGCCATTCT
TTTTTTTTTAATTCCCTCGCTGGCATTTTTATTTACTTTTTCATTTTTTAAAATCTAAAA
TATCCCAATGCAAAATATGTGCCGTTTCTGCAAAAAGTGCCAAACACCTTGCCGTTTATC
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAA
AGTTATTAAAAA
   
Sequence Name 07-K118836-0022-302-A10C-8409
GenBank Accession LN489490 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K118836-0022-302-A10C-8409
ATTTACGATAAACGGCAAGGTGTTTGGCACTTTTTGCAGAAACGGCACATATTTTGCATT
GGGATATTTTAGATTTTAAAAAATGAAAAAGTAAATAAAAATGCCAGCGAGGGAATTAAA
AAAAAAAGAATGGCAGTTGTTAGGTTTAAATTTTATAAAGGAGGAAGCCACGTTTTGCGT
TTAGTCGCCACCCAAAAAAAACCCTCAAGTCTTCTTCTTCTTCCTCAATCTCTCCACCTC
TTTTCAAAA

Last Updated on 06.12.2018 16:11