Genotyping and primer details


Genotyping and primer details for 302A10 - At5g56940
Line ID 302A10
Locus At5g56940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23032267 of line 302A10 Chr5:23032267
   
Insertion allele amplicon 8474 - AR15
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AR15: 5'-GTTTGAAAAGAGGTGGAGAGATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23032543
   
Insertion allele amplicon 8474 - BV09
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BV09: 5'-TATAACAAATGAAACTTCTCCGCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23031589
   
wt amplicon (over InsPos) AR15 - BV09
wt amplicon start Chr5:23031589
wt amplicon end Chr5:23032543
wt amplicon size 955 bp
Locus specific primer BV09: 5'-TATAACAAATGAAACTTCTCCGCC-3'
2nd locus specific primer AR15: 5'-GTTTGAAAAGAGGTGGAGAGATTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11