Sequences from confirmation amplicon


Confirmation sequences for line 302B09 and hit At1g30010 are shown.
Line ID 302B09
Locus At1g30010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10514891
Primers used for PCR 12sf - 8474, show primer details
   
Sequence Name 42-K116491-0022-302-B09C-12sf
GenBank Accession LN504600 [GenBank]
Sequencing primer gene specific primer, 12sf: 5'-AGTTCAATGATTTGTTGGGTAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K116491-0022-302-B09C-12sf
GGATTAAAAAGAATAGCGATGAAATTCTCCGCTATAACTCTCAGTCTCTGGTTTATCATG
ATCCTTGTACCAGATACACCAAATAAAGGAGGTAATGGTAGACAATCATGTTTGTGAGTC
TAATTTCTGCAACCCATCATCTGCTTCTCTTTTATTTTCATACTTTGATCTCCAAGCACC
AAGAGTCTTGAAATCCCACACAGCCTCTGAGATCTCATCCTGGGGCAAGGTTAGACCCTC
GCGTTTTATTGACCTATGCAACCCACAGAAGAATTTAGGGTCTTGTAGCCTGATGTACTC
CTGCAAAACTTGCTCATGATTTGGGATCCAGTTCCTTGGGAATGGAGTATAATCACAAGA
CGGAATCAAAGACATGCGAGAGAATTGAACTCCTTCTATAGCATCAACGAGTCCCATTCT
CAGAAGATCAGAGTATTCAGGTGCAGAATTATTGCTACTCTCGCTTAATGGATGGCTAAG
ATCCCGTGAGGCAATGCTGTACACTTTGGCACGAGATTTAAGTCTATACCTTGCAGCGTA
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTATATGTC
CGCA
   
Sequence Name 10-K116490-0022-302-B09C-8409
GenBank Accession LN490820 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K116490-0022-302-B09C-8409
GCCATTTACATTGTACGCTGCAAGGTATAGACTTAAATCTCGTGCCAAAGTGTACAGCAT
TGCCTCACGGGATCTTAGCCATCCATTAAGCGAGAGTAGCAATAATTCTGCACCTGAATA
CTCTGATCTTCTGAGAATGGGACTCGTTGATGCTATAGAAGGAGTTCAATTCTCTCGCAT
GTCTTTGATTCCGTCTTGTGATTATACTCCATTCCCAAGGAACTGGATCCCAAATCATGA
GCAAGTTTTGCAGGAGTACATCAGGCTACAAGACCCTAAATTCTTCTGTGGGTTGCATAG
GTCAATAAAACGCGAGGGTCTAACCTTGCCCCAGGATGAGATCTCAGAGGCTGTGTGGGA
TTTCAAGACTCTTGGTGCTTGGAGATCAAAGTATGAAAATAAAAGAGAAGCAGATGATGG
GTTGCAGAAATTAGACTCACAAACATGATTGTCTACCATTACCTCCTTTATTTGGTGTAT
CTGGTACAAGGATCATGATAAACCAGAGACTGAGAGTTATAGCGGAGAATTTCATCGCTA
TTCTTTTTTAATCCCCTTCTTCGCAAGAGACCCTGATTCTGGATCTGGCTTCCTTACCCA
A

Last Updated on 06.12.2018 16:11