Sequences from confirmation amplicon


Confirmation sequences for line 302H04 and hit At1g54960 are shown.
Line ID 302H04
Locus At1g54960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20500451
Primers used for PCR PA72 - 8474, show primer details
   
Sequence Name 20-K037017-022-302-H04C-PA72
GenBank Accession LN495125 [GenBank]
Sequencing primer gene specific primer, PA72: 5'-ATGGCATGATTATGAAGGTACTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K037017-022-302-H04C-PA72
CCTTTTTCAACAGTTTAAAGATGTTACCATCATCAACCTTTCAGGAAACCCAATAGTTTT
TCAACAGAGCGACTTACAGATTCAGGAAAGGCTCCAAATTTCTCCAAGAGTGAAGATATA
GATCCACCAGGAACAAATTCAAGCAAGATATTCAAAGTTTCATCTTCCCTCACCGTACCC
AAATATCTCTAAGGCCAAACAAAGAATGCAAAAGTTATAAAAAAGAAAACACAAACACAC
TGAATCAATCAGCATTGCAACAAAACAATAACGTTATTGCTTACAACTATATTTGGATGA
GAGAGATTCTTGAGTAGCTTCACTTCCTCTTCAAGCTCCTGAATATGAGCCTTTTGTTCA
CCAAAATAAGAAAGTCATGTAATGTAAAAAAAATGTAGCACAATATCAACAAGAAACAAA
TAGGCTTGTTTATGAACCTGAGTTTTTTCCTTGGATGCACAATTAGATGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 19-K037017-022-302-H04C-8409
GenBank Accession LN494671 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K037017-022-302-H04C-8409
CATTTACATCTAATTGTGCATCCAGGAAAAAACTCAGGTTCATACACAAGCCTATTTGTT
TCTTGTTGATATTGTGCTACATTTTTTTTACATTACATGACTTTCTTATTTTGGTGAACA
AAAGGCTCATATTCAGGAGCTTGAAGAGGAAGTGAAGCTACTCAAGAATCTCTCTCATCC
AAATATAGTTGTAAGCAATAACGTTATTGTTTTGTTGCAATGCTGATTGATTCAGTGTGT
TTGTGTTTTCTTTTTTATAACTTTTGCATTCTTTGTTTGGCCTTAGAGATATTTGGGTAC
GGTGAGGGAAGATGAAACTTTGAATATCTTGCTTGAATTTGTTCCTGGTGGATCTATATC
TTCACTCTTGGAGAAATTTGGAGCCTTTCCTGAATCTGTAAGTCGCTCTGTTGAAAAACT
ATTGGGTTTCCTGAAATGTTGATGATGGTAACATCTTTAAACTGTTTGAAAAAGGTTGTT
CGGACATACACGAACCAACTGCTTTTGGGATTGGAGTACCTTCTAATCCATGCC

Last Updated on 06.12.2018 16:11