Sequences from confirmation amplicon


Confirmation sequences for line 304B04 and hit At1g60140 are shown.
Line ID 304B04
Locus At1g60140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22179717
Primers used for PCR FQ78 - 8474, show primer details
   
Sequence Name 86-K033881-022-304-B04C-FQ78
GenBank Accession LN514610 [GenBank]
Sequencing primer gene specific primer, FQ78: 5'-ATTCATACTCCAATCCAAGCATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K033881-022-304-B04C-FQ78
CCGTGCATAATCAAACGTGTGAAAACCAATTAAGTCACAATTTAAGAGCCCCCTTAATAG
TTCCTCTCGAACAGGTAAGGTTCGATAAACTTCAAAAGAAGGGAATGGACTGTGGAGGAA
GAAACCAAGCTTAACCCTGTGAAACCGCCTTCTCAAAAATGTTGGGAGAACCATTAAATG
ATAGTCATGAATCCAGATATAATCTTCTTCAAGATTAATCACACCCATTACCTTATCTGC
AAATATCTTGTTGGCAGAAACATATGCCTGCCAAAGACCACGATCAAACCGTTCACCATG
ATCAGGACACATGGGTAACATGTAATGGAAAAGTGGCCATAGTTGCTGTTTACAAAAGCC
AAGGTAGAATTTTTTATGCACATCTTGTGGGAGGAAAGTCGCTACACAATTGAATTCCTC
AAAAAGATTATGGGAAACCTCGTCTATTAGCAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAAAATGG
   
Sequence Name 30-K033881-022-304-B04C-8409
GenBank Accession LN499622 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K033881-022-304-B04C-8409
TGAGCCATTGCTAATAGACGAGGTTTCCCATAATCTTTTNTGAGGAACCCAAAAATGTAG
CGACTTTCCTCCCACAAGATGTGCATACAAAAAACTACCTTGGCTTTTGTAAACAGCAAC
TATGGCCACTTTTCCATTACATGTTACCCATGTGTCCTGATCATGGTGAACGGTTTGATC
GTGGTCTTTGGCAGGCATATGTTTCTGCCAACAAGATATTTGCAGATAAGGTAATGGGTG
TGATTAATCTTGAAGAAGATTATATCTGGATTCATGACTATCATTTAATGGTTCTCCCAA
CATTTTTGAGAAGGCGGTTTCACAGGGTTAAGCTTGGTTTCTTCCTCCACAGTCCATTCC
CTTCTTCAGAAATTTATCGAACCTTACCTGTTCGAGAGGAACTACTAAGAGGGCTCTTAA
ATTGTGACTTAATTGGTTTTCACACGTTTGATTATGCACGGCATTTCTTGTCATGCTGCT
GTAGAATGCTTGGATTGAANTATGA

Last Updated on 06.12.2018 16:11