Sequences from confirmation amplicon


Confirmation sequences for line 304B11 and hit At1g74060 are shown.
Line ID 304B11
Locus At1g74060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27850381
Primers used for PCR 68yh - 8474, show primer details
   
Sequence Name 34-K122428-0022-304-B11C-68yh
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 68yh: 5'-GTCTCTTCTTGATTATCGTCCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K122428-0022-304-B11C-68yh
GCTAAGGTCAACAGGAATCCTGATCTGATCAGGGGAGTTGGTAAATACTCAAGGTCTCAG
ATGTACCATAAGAGAGGTTTGTGGGCAATCAAGGCCAAAAATGGAGGCGTTTTCCCCCGC
CACGACGCTAAATCTAAGGTTGATGCTCCGGTGGAGAAGCCACCGAAGTTCTATCCAGCT
GAAGACGTGAAGAAACCTCTCCCCAACAGGCGCACGGCAAAACCAGCCAAGCTCAGGTAC
TTAGCTGATACACTGTCTCATTGTCTTGGAGTCTGAAATCTAGCTCAATGCAATAGTTGT
TTTCTTTGGTTCTTAAGGAGTGCTTTGTATGCATTTAAATCTTTGAATCTCCCGAAATTT
TTTGACTAGCTTAGTGTTTTGAAATCGTTAATTAGTCATTGTGGTCTGTTCTATTTGGTT
TGTGAATTTATCTTGATTTCTGTGGAAAGTTTGCCTCTTTTGCTTGTATTTTGTGTTACA
TTGTTCGGAATTTGTTATGTTGTAATCCGATATTCATATTTCTCTATTGCAGAGCTAGCA
TCACTCCAGGGACAGTGCTTATCATCCTTGCTGGTAGATTTAAGGGCAAGAGAGTTGTCT
TCCTTAAGCAGCTTGCCTCCGGTTTGCTTCTTGTGACTGGTGAGTTTTATGCTAATCTAC
TTTCTTTATTTGCCACATTCTTTATTCGAAATGCAGAACGCCCATTCACTAAACTTGCTT
TCTGTATTCTGTATAAAGTTGACATTCCTGTTTTGTTATTGACCTTAGGACCATTCAAGA
TCAATGGTGTTCCTCTGAGACGTGTTAACCAGGCCTATGTCATTGGTACTTCCACAAAGG
TTGACATTTCTGGAGTTACCCTCGACAAATTCGATGATAAGTACTTCGGCAAGGTTGC
   
Sequence Name 95-K122444-0022-304-B11C-8474
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>95-K122444-0022-304-B11C-8474
ATGGTATTACTCTTTCTTTTTCTCATATTTCTTTTTCTCATTGCTTTTAATTTGGGTAGT
GTATAAAGGAGAGAACTTTAATCGTCTATTACAAAATGAGAACCAAATGACTCTTAGAGA
AAGACAAGAAGAATGTTTGGGATACCTCTTTCTCAGCCTCGAAGAACTCTCCTTCAGTCT
TCTTCTTTTTCTTCTCAGCAACCTTGCCGAAGTACTTATCATCGAATTTGTCGAGGGTAA
CTCCAGAAATGTCAACCTTTGTGGAAGTACCAATGACATAGGCCTGGTTAACACGTCTCA
GAGGAACACCATTGATCTTGAATGGTCCTAAGGTCAATAACAAAACAGGAATGTCAACTT
TATACAGAATACAGAAAGCAAGTTTAGTGAATGGGCGTTCTGCATTTCGAATAAAGAATG
TGGCAAATAAAGAAAGTAGATTAGCATAAAACTCACCAGTCACAAGAAGCAAACCGGAGG
CAAGCTGCTTAAGGAAGACAACTCTCTTGCCCTTAAATCTACCAGCAAGGATGATAAGCA
CTGTCCCTGGAGTGATGCTAGCTCTGCAATAGAGAAATATGAATATCGGATTACAACATA
ACAAATTCCGAACAATGTAACACAAAATACAAGCAAAAGAGGCAAACTTTCCACAGAAAT
CAAGATAAATTCACAAACCAAATAGAACAGACCACAATGACTAATTAACGATTTCAAAAC
ACTAAGCTAGTCAAAAAATTTCGGGAGATTCAAAGATTTAAATGCATACAAAGCACTCCT
TAAGAACCAAAGAAAACAACTATTGCATTGAGCTAGATTTCAGACTCCAAGACAATGAGA
CAGTGTATCAGCTAAGTACCTGAGCTTGGCTGGTTTTGCCGTGCGCCTGTTGGGGAGAGT
TTCTTCACGTCTTCAGCTGGATAGAACTTCGGTGGCTTCTC

Last Updated on 06.12.2018 16:11