Sequences from confirmation amplicon


Confirmation sequences for line 304E12 and hit At3g63470 are shown.
Line ID 304E12
Locus At3g63470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23439373
Primers used for PCR 53qa - 8474, show primer details
   
Sequence Name 11-K115511-0022-304-E12C-53qa
GenBank Accession LN491256 [GenBank]
Sequencing primer gene specific primer, 53qa: 5'-GGCGATTCTAAGAACAAGACATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K115511-0022-304-E12C-53qa
TATTTTTTATCACAGAATCGTTTACCATTAATTTATTTTTGTTATTTAATAAAAAAGAAA
AACGATAGGTACCATTGTTCCATGCATATCGGTTTCTAAAAAGCGTTTTGCCGTCGCTGT
GTACTCTGAACGGCCCAAGCTCTTGTAATGCTCCATACGCCAGTGACGAGCATCCCGGTC
CTGTTAATTATTTCCAACACACTTTATGCATATGTATGTATTATTAGTCACCCATATTTC
TGTAATCAAATTCTCGATTTTGTGACAGCTAATTCACTGATGAGCTTACTATTAACGTCT
CTGGTGAATTTGAATATATGTTGTACATTGCATTTTATTTTTATGTACATACATAATATA
GAAATCAGTATATAATATATAGATAGATACGTAGTGGCTGGCTAAATAATTGACCAGTAG
AGCTGTGCAACAAACAAAGCACGCCGAGAAATCCTCAGCAATCTACATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 03-K115510-0022-304-E12C-8409
GenBank Accession LN487619 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K115510-0022-304-E12C-8409
ATTGCTGAGGATTTCTCGGCGTGCTTTGTTTGTTGCACAGCTCTACTGGTCAATTATTTA
GCCAGCCACTACGTATCTATCTATATATTATATACTGATTTCTATATTATGTATGTACAT
AAAAATAAAATGCAATGTACAACATATATTCAAATTCACCAGAGACGTTAATAGTAAGCT
CATCAGTGAATTAGCTGTCACAAAATCGAGAATTTGATTACAGAAATATGGGTGACTAAT
AATACATACATATGCATAAAGTGTGTTGGAAATAATTAACAGGACCGGGATGCTCGTCAC
TGGCGTATGGAGCATTACAAGAGCTTGGGCCGTTCAGAGTACACAGCGACGGCNAAACGC
TTTTTAGAAACCGATATGCATGGAACAATGGTACCTATCGTTTTTCTTTTTTATTAAATA
ACAAAAATAAATTAATGGTAAACGATTCTTGTGATTAAAAAATTATCAAACGGGTGGTGG
TGGTGCAGCTGCGAATGTCTTGTTCTAANAATCGC

Last Updated on 06.12.2018 16:11