Sequences from confirmation amplicon


Confirmation sequences for line 304F02 and hit At3g50140 are shown.
Line ID 304F02
Locus At3g50140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18592659
Primers used for PCR 13wj - 8474, show primer details
   
Sequence Name 64-K118585-0022-304-F02C-13wj
GenBank Accession LN511297 [GenBank]
Sequencing primer gene specific primer, 13wj: 5'-GTTTTGTTCACTTTTTGTTCCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K118585-0022-304-F02C-13wj
CATCGTTGCCTATTTATCAATTCATAATGATTTTGCTATTTAGGCATATACATTTTTTCC
CGCCTAGAGAACCCTCATAATTCCTTAAACTCACAAAAACACTATATGGTCTCTCTTCGT
GTTTTTTGTAGGTACCAAGTCTCTTTTCTCAAATCTTATAGCTTACGAGCAGTGCCATAT
CGATTCAACGAACGACATAACATCCTACATTATCTTCATGGACAATCTAATAGATTCTGC
TGAAGATATTAGGTATTTGCATTACTATGATATCATCGAGCACTGGTTAGGAAATGATTC
AGAAGTTGCTGATGTCTTCAACCGGCTATGTCAAGAAGTGGCTTTCGACCTCGAAAATAC
TTACTTATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 34-K118584-0022-304-F02C-8409
GenBank Accession LN501604 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K118584-0022-304-F02C-8409
GCCATTTACAATTGAATTATCCTGATAAGTAAGTATTTTCGAGGTCGAAAGCCACTTCTT
GACATAGCCGGTTGAAGACATCAGCAACTTCTGAATCATTTCCTAACCAGTGCTCGATGA
TATCATAGTAATGCAAATACCTAATATCTTCAGCAGAATCTATTAGATTGTCCATGAAGA
TAATGTAGGATGTTATGTCGTTCGTTGAATCGATATGGCACTGCTCGTAAGCTATAAGAT
TTGAGAAAAGAGACTTGGTACCTACAAAAAACACGAAGAGAGACCATATAGTGTTTTTGT
GAGTTTAAGGAATTATGAGGGTTCTCTAGGCGGGAAAAAATGTATATGCCTAAATAGCAA
AATCATTATGAATTGATGAAATAGGCAACGATTGATGTCAGTCTATGTACTTTAGCAACT
TATGGGAACAAAAGTGAAACAAAAC

Last Updated on 06.12.2018 16:11