Sequences from confirmation amplicon


Confirmation sequences for line 305G03 and hit At1g50560 are shown.
Line ID 305G03
Locus At1g50560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18725846
Primers used for PCR 49ux - 8474, show primer details
   
Sequence Name 31-K117337-0022-305-G03C-49ux
GenBank Accession LN500320 [GenBank]
Sequencing primer gene specific primer, 49ux: 5'-AAGACAGCAAGTTCCATTACCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K117337-0022-305-G03C-49ux
CTCCTAGATTGACGGTAGCTAGATGGTTAATTCCGAAAATACCCAAATCTAGTTCCATTA
TCAGTTTGAGACATATGTCTGCAACTTCAACAAGACATATCATACTCCTCCATCATCAAC
TAAGGATCAAAGCTAGCTGGGTTGATTCGAGCAACAGGAATGCATTTAAGTGGATGAGCC
ATGGTCAGCGTCATTTCTCCAGCGCACATGGATCAGCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 26-K117336-0022-305-G03C-8409
GenBank Accession LN498105 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K117336-0022-305-G03C-8409
CGCTGACCATGGCTTCATCCCACTTAAACGCATTCCTGTTGCTTCGAATCAACCCAGCTA
GCTTTTGATCCTTAGTTTGATGATGGAGGAGTATGATATGTCTTGTTGAAGTTGCAGACA
TATGTCTCAAACTGATAATGGAACTAGATTTGGGTATTTTCGAATTAACCATCTAGCTAC
CGTCAATCTAGAGATCCTTACATGATGGTTGAATTTGATATATCCTGTTGATGTTGAAGA
CATATATCTCTGCAACTGGTAATG

Last Updated on 06.12.2018 16:11