Sequences from confirmation amplicon


Confirmation sequences for line 305G03 and hit At3g44680 are shown.
Line ID 305G03
Locus At3g44680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16229476
Primers used for PCR HF22 - 8474, show primer details
   
Sequence Name 35-K032416-022-305-G03C-8409
GenBank Accession LN501717 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K032416-022-305-G03C-8409
ATACTTATTACAGGGTTTTAGGTGAAGTTTCTGATTTTTTCGATTCTTTGGCCTGAGTTT
AAGCTTAAGATAGAAGCCTTCTTGTGGTGAAGACGACTCTAGTACCTGTGAAGTTGACTT
CTTTGATTGGTTTGGTCAAGTCTTTGTTATTTCTTTGCTAAAATATGAAACCCTAGAACT
TTTGTTAAATTTGGCATTGCATATAATGTTTGAAATTGTGTTCTTTCGAGATTCCTCTCT
GAGAAACTTTTATAGACTTGATAGTTATATAACTTTCATTTTCGTACTTCAGATTTTCTG
CANNATTCAAA
   
Sequence Name 35-K032416-022-305-G03C-HF22
GenBank Accession LN501718 [GenBank]
Sequencing primer gene specific primer, HF22: 5'-GTTTGAATCATGCAGAAAATCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K032416-022-305-G03C-HF22
TACTATCAAGTCTATAAAAGTTTCTCAGAGAGGAATCTCGAAAGAACACAATTTCAAACA
TTATATGCAATGCCAAATTTAACAAAAGTTCTAGGGTTTCATATTTTAGCAAAGAAATAA
CAAAGACTTGACCAAACCAATCAAAGAAGTCAACTTCACAGGTACTAGAGTCGTCTTCAC
CACAAGAAGGCTTCTATCTTAAGCTTAAACTCAGGCCAAAGAATCGAAAAAATCAGAAAC
TTCACCTAAAACCCTTGTAATAAGTATATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
ATCTTTATTTCGACGTGTCTACATTCA
Primers used for PCR FQ05 - 8474, show primer details
   
Sequence Name 52-K035338-022-305-G03C-8409
GenBank Accession LN507733 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K035338-022-305-G03C-8409
GCCATTTACTTATTCTAGACTATAGATATGAAAGATTTCACCTTTCGATGCATCGATTGA
GCAATAAAGACAAAAACTAGTGCGGTTCAAGTGGATAAAACTTGCTCAAGAGTTACATCA
ATCTCAAGGATTTTGGAGTTTCTTTACCATAAATAAAGAAGAAAAGGGAAAGAAATGAAT
ATACCATCATAAAAGTAAGAGATTTTGTCCTTGGAACGCATCTCTTAAAGAAGAAAAGAC
ACGAAGATCGT
   
Sequence Name 52-K035338-022-305-G03C-FQ05
GenBank Accession LN507734 [GenBank]
Sequencing primer gene specific primer, FQ05: 5'-ATTTCGTACGATCTTCGTGTCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K035338-022-305-G03C-FQ5
GTTCAGGACAAATCTCTTACTTTTATGATGGTATATTCATTTCTTTCCCTTTTCTTCTTT
ATTTATGGTAAAGAAACTCCAAAATCCTTGAGATTGATGTAACTCTTGAGCAAGTTTTAT
CCACTTGAACCGCACTAGTTTTTGTCTTTATTGCTCAATCGATGCATCGAAAGGTGAAAT
CTTTCATATCTATAGTCTAGAATAAGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGG
AAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGGG

Last Updated on 06.12.2018 16:11