Sequences from confirmation amplicon


Confirmation sequences for line 306A08 and hit At1g13890 are shown.
Line ID 306A08
Locus At1g13890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4752492
Primers used for PCR IW55 - 8474, show primer details
   
Sequence Name 21-K119249-0022-306-A08C-IW55
GenBank Accession LN495882 [GenBank]
Sequencing primer gene specific primer, IW55: 5'-TTTCTTCGATACGAAAATGTGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K119249-0022-306-A08C-IW55
TAGAAAATGTTTGTGAGATCAGGGGGAGAAGCTTTTGAACAACTTAGGAGGAATGTTTTC
AAAGCCATGGAAACCAAAGAAGACTAAAAATATCACAGGACCTATGATCACACCAGGTTT
TAAAGCTTCCGAGCTCTTGATATTGATTGGATAAAATAATGTTATGATGATCATGTATCT
TATAATATCTTAGAGAAGGGTTGATTCATACCTAGCCCGTTAATAACTCATATAATCTCA
ATCCTTATTGTATTCACGTTGTTAGGACTCTTGATCATGTGTATGGTGGTCATGTTTAAT
ATAGTTATCGAAATTTTAGATAAACCGTCGAAGAAGAGCGAGAATCACAAGGAAGAGCGG
GAGAAACTTGGTTTGGGTGCAAAAGGACGATCGAGCAGTCAACCAGCATTAGATCAACCA
ACAAATGCGTTGCAGAAAGTTGAGGTTTGTGTTCTTGTGGTGTGATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTTA
TT
   
Sequence Name 05-K119248-0022-306-A08C-8409
GenBank Accession LN488576 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K119248-0022-306-A08C-8409
ATTGAATATCACACCACAAGAACACAAACCTCAACTTTCTGCAACGCATTTGTTGGTTGA
TCTAATGCTGGTTGACTGCTCGATCGTCCTTTTGCACCCAAACCAAGTTTCTCCCGCTCT
TCCTTGTGATTCTCGCTCTTCTTCGACGGTTTATCTAAAATTTCGATAACTATATTAAAC
ATGACCACCATACACATGATCAAGAGTCCTAACAACGTGAATACAATAAGGATTGAGATT
ATATGAGTTATTAACGGGCTAGGTATGAATCAACCCTTCTCTAAGATATTATAAGATACA
TGATCATCATAACATTATTTTATCCAATCAATATCAAGAGCTCGGAAGCTTTAAAACCTG
GTGTGATCATAGGTCCTGTGATATTTTTAGTCTTCTTTGGTTTCCATGGCTTTGAAAACA
TTCCTCCTAAGTTGTTCAAAAGCTTCTCCCCCTGATCTCACAAACATTTTTTCTTATCAA
CGCTCCATAAAACAGAACATATAATTTCACATTTTCGTATCGAAGAAAAANATGNTTTCA
AGAGTTTTGGCTTCCT

Last Updated on 06.12.2018 16:11