Sequences from confirmation amplicon


Confirmation sequences for line 307A11 and hit At3g54140 are shown.
Line ID 307A11
Locus At3g54140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20046821
Primers used for PCR 03up - 8474, show primer details
   
Sequence Name 49-K117497-0022-307-A11C-03up
GenBank Accession LN506968 [GenBank]
Sequencing primer gene specific primer, 03up: 5'-CTGGATACAAATGAATGTTGGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K117497-0022-307-A11C-03up
CGCGGTTTGCTTTTTCTTCTTCGGAAGCCGTTTTTACAGACTTCAGAGACCTGGAGGGAG
TCCACTTACTAGGATCTTTCAGGTTATAGTAGCGGCTTTTCGGAAGATAAGTGTTAAGGT
TCCAGAGGACAAGTCTCTGCTCTTTGAAACTGCAGATGATGAGAGTAACATCAAAGGTAG
CCGGAAACTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCG
   
Sequence Name 17-K117496-0022-307-A11C-8409
GenBank Accession LN494038 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K117496-0022-307-A11C-8409
TTTACATTGAATATATCCTGCAGTTTCCGGCTACCTTTGATGTTACTCTCATCATCTGCA
GTTTCAAAGAGCAGAGACTTGTCCTCTGGAACCTTAACACTTATCTTCCGAAAAGCCGCT
ACTATAACCTGAAAGATCCTAGTAAGTGGACTCCCTCCAGGTCTCTGAAGTCTGTAAAAA
CGGCTTCCGAAGAAGAAAAAGCAAACCGCGATAACCATCGCGACTGTTGGAACACCGAAA
CCCCATCCCCAACCAACATTCATTTGTATCCAG

Last Updated on 06.12.2018 16:11