Sequences from confirmation amplicon


Confirmation sequences for line 308B09 and hit At5g45530 are shown.
Line ID 308B09
Locus At5g45530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18456440
Primers used for PCR 22hw - 8474, show primer details
   
Sequence Name 51-K112758-0022-308-B09C-22hw
GenBank Accession LN507554 [GenBank]
Sequencing primer gene specific primer, 22hw: 5'-TCGTCGGTTAAGAAGATTTCAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K112758-0022-308-B09C-22hw
GATTCTTCATTAGCTAGCGTAGTTGAACCAGCGTCTGCTCGTTGAAGCATTCTGTCTTTG
AATTTGTCTAAGCTCGCTGAGTACAATGCTCGTGTCCTCTCCACGTACTTAATCGTTCCT
GTGATGAATAAAAGTGTAATTGGTGGCCATAATACGTTTTCTAGCGATTGCACCACCGCA
TAAACACCTGAAAACGACATAAAAATGTTATGAGAAAACCTTCATAATGAGGCTATTTAA
ATTTTTTCTAATTAATAAAACGGTAAAATATATTTTGATTATTTTGGATATTGATTTTTC
TACTCAAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCG
   
Sequence Name 11-K112757-0022-308-B09C-8409
GenBank Accession LN491188 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K112757-0022-308-B09C-8409
ATTTACTTTGAGTAGAAAATAATATCCAAATAATCAAAATATATTTTACCGTTTTATTAA
TTAGAAAAAATTTAAATAGCCTCATTATGAAGGTTTTCTCATAACATTTTTATGTCGTTT
TCAGGTGTTTATGCGGTGGTGCAATCGCTAGAAAACGTATTATGGCCACCAATTACACTT
TTATTCATCACAGGAACGATTAAGTACGTGGAGAGGACACGAGCATTGTACTCAGCGAGC
TTAGACAAATTCAAAGACAGAATGCTTCAACGAGCAGACGCTGGTTCTAACTACGCTAAG
CTAATGGAAGAATTCGCTTCAAGAAAAATGTCAAATCTCCCAACTGAAATCTTCTTAACC
GACG

Last Updated on 06.12.2018 16:11