Sequences from confirmation amplicon


Confirmation sequences for line 308B10 and hit At2g02220 are shown.
Line ID 308B10
Locus At2g02220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:586101
Primers used for PCR EQ16 - 8474, show primer details
   
Sequence Name 34-K102871-0022-308-B10C-EQ16
GenBank Accession LN501372 [GenBank]
Sequencing primer gene specific primer, EQ16: 5'-TCCCAACAAGTATAAATCTCCCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K102871-0022-308-B10C-EQ16
AATTCATGGGTCGCTTCCGTCTCATATCTGCCATAACTCTACCCATAATAGGGTTGTGAA
ACTTGCGGTGAACTACTTCGCCGGCAACTTCACTTCCGGGTTTGGGAAATGTGTCTTGCT
TGAGCATCTCTGTCTTGGTATGAACGATCTTACTGGTAACATCCCTGAGGATTTGTTTCA
TCTCAAAAGATTGAATCTTTTAGGGATTCAAGAGAATCGTCTCTCTGGTTCGTTGAGTCG
TGAGATTAGGAATCTCTCAAGTCTTGTTCGTCTTGATGTTTCTTGGAATTTGTTTTCCGG
TGAAATCCCTGATGTGTTCGACGAATTGCCTCAGTTAAAGTTTTTCTTAGGTCAGACCAA
TGGATTCATTGGAGGAATACCTAAATCGTTGGCGAATTCACCGAGTTTGAATCTGCTTAA
CTTGAGGAACAATTCTTTATCGGGTCGTTTGATGTTGAATTGTACGGCGATGATTGCTTT
GAACTCTCTTGATTTAGGTACCAATAGATTCAATGGGAGGTTACCTGAGAATCTACATGG
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
TTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
ACATTCACGTCCAAATGGGA
Primers used for PCR KH51 - 3144, show primer details
   
Sequence Name 07-K106971-0022-308-B10C-3144
GenBank Accession LN489293 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K106971-0022-308-B10C-3144
ATCCACTATCCTTGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTAAA
GAACGTTAACCTCGCGAGGAACACCTTCCATGGACAAGTACCAGAGAGTTTCAAGAACTT
CGAGAGCTTATCTTACTTCTCGTTATCGAATTCGAGTTTGGCTAATATCTCTTCAGCGCT
TGGGATACTTCAGCATTGCAAGAACTTGACGACTTTGGTTCTTACATTGAATTTCCATGG
AGAGGCTTTACCCGATGATTCAAGTCTTCATTTCGAGAAGCTTAAGGTGCTTGTAGTGGC
GAATTGTAGGCTTACTGGTTCGATGCCGAGGTGGTTAAGCTCGAGTAATGAACTTCAGTT
GTTGGATCTTTCTTGGAACCGTTTAACCGGCGCTATCCCGAGCTGGATTGGTGACTTCAA
GGCTCTGTTCTACTTGGATTTATCTAACAACTCGTTTACAGGAGAGATCCCTAAGAGCTT
AACTAAGTTAGAGAGTCTCACTAGCCGTAATATCTCAGTCAAATGAGC
   
Sequence Name 23-K106972-0022-308-B10C-KH51
GenBank Accession LN496575 [GenBank]
Sequencing primer gene specific primer, KH51: 5'-TGGCTCATTGACTGAGATATTACG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K106972-0022-308-B10C-KH51
TAGCTCTAGGGATCTCTCCTGTAACGAGTTGTTAGATAAATCCAAGTAGAACAGAGCCTT
GAAGTCACCAATCCAGCTCGGGATAGCGCCGGTTAAACGGTTCCAAGAAAGATCCAACAA
CTGAAGTTCATTACTCGAGCTTAACCACCTCGGCATCGAACCAGTAAGCCTACAATTCGC
CACTACAAGCACCTTAAGCTTCTCGAAATGAAGACTTGAATCATCGGGTAAAGCCTCTCC
ATGGAAATTCAATGTAAGAACCAAAGTCGTCAAGTTCTTGCAATGCTGAAGTATCCCAAG
CGCTGAAGAGATATTAGCCAAACTCGAATTCGATAACGAGAAGTAAGATAAGCTCTCGAA
GTTCTTGAAACTCTCTGGTACTTGTCCATGGAAGGTGTTCCTCGCGAGGTTAACGTTCTT
TAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGT
CCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGA
TTGTGCGTCATCCCTTACGTCAGTGGAGATATC

Last Updated on 06.12.2018 16:11