Sequences from confirmation amplicon


Confirmation sequences for line 308C04 and hit At2g40400 are shown.
Line ID 308C04
Locus At2g40400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16871864
Primers used for PCR AB56 - 8474, show primer details
   
Sequence Name 03-K105708-0022-308-C04C-AB56
GenBank Accession LN487488 [GenBank]
Sequencing primer gene specific primer, AB56: 5'-ATCCATACTTGCCTCTTCAGTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K105708-0022-308-C04C-AB56
ATGGCTGCACCTCGGGGATTTGGTTGGAAGTTGTGACACTTTTCTGAGTTTGCAGACCCG
TAGAGCGCGCAAGATCAATCCATTGCTGCGTTTTTATGAAAAAAGTTGGAATGAAGTTAA
ACGGTATATCAAGAATTAACCAAAAATAATAATAAGGAAAAAGGAGACAAAGAATTCAAG
AAGAGATCATAGTACAGGCCTAATTCTGACCTGAGTTCCAAAGTAGGAGTTAGCTACTCG
AACAACAAATGAAATCATGTTTACAAGTAGAGGTTGAGAAGAAAGCTCGTCAGAAATGCG
ATGCTCTATTAACCCAGCAATTATCTGCAATTGAAAGTAATATGATTTTAGAGGCATAAA
TTCAAAGCATACTGTTCTTCATCAAAAGGCCTTCCTTGGCTAAGATGCAAAAGCATAATA
TATTGCTGTGGTCATTAAGCAACTTTAACTACTTTTCATTAATATCCCTCATTCATTGAG
TCAACATGTAAAGCACCGCGCATCACCATACCTGATAACGGATATTCGAAGATGTTCCCA
GATAACCTCCATACACAAGGGCCGTCTTCAGCATAGGAGACCTCTTTGCTTGCTCACCAA
CACCCAACTCTGGTTTAATGAATTTTCGTATTGCATACAAAGCATTTGAAGATCCCACAG
CTGCAAAACTGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 08-K105709-0022-308-C04C-8409
GenBank Accession LN489731 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K105709-0022-308-C04C-8409
TGCTTTGTATGCAATACGAAAATTCATTAAACCAGAGTTGGGTGTTGGTGAGCAAGCAAA
GAGGTCTCCTATGCTGAAGACGGCCCTTGTGTATGGAGGTTATCTGGGAACATCTTCGAA
TATCCGTTATCAGGTATGGTGATGCGCGGTGCTTTACATGTTGACTCAATGAATGAGGGA
TATTAATGAAAAGTAGTTAAAGTTGCTTAATGACCACAGCAATATATTATGCTTTTGCAT
CTTAGCCAAGGAAGGCCTTTTGATGAAGAACAGTATGCTTTGAATTTATGCCTCTAAAAT
CATATTACTTTCAATTGCAGATAATTGCTGGGTTAATAGAGCATCGCATTTCTGACGAGC
TTTCTTCTCAACCTCTACTTGTAAACATGATTTCATTTGTTGTTCGAGTAGCTAACTCCT
ACTTTGGAACTCAGGTCAGAATTAGGCCTGTACTATGATCTCTTCTTGAATTCTTTGTCT
CCTTTTTCCTTATTATTATTTTTGGTTAATTCTTGATATACCGTTTAACTTCATTCCAAC
TTTTTTCATAAAAACGCAGCAATGGATTGATCTTGCGCGCTCTACGGGTCTGCAAACTCA
GAAAAGTGTCACAACTTCCAACCAAATCCCCGAGGTTGCGAGCCAATCCACAGTGGAATA
CAGTACAACTGAAGAG

Last Updated on 06.12.2018 16:11