Sequences from confirmation amplicon


Confirmation sequences for line 308F08 and hit At3g30775 are shown.
Line ID 308F08
Locus At3g30775 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:12450004
Primers used for PCR MR52 - 8474, show primer details
   
Sequence Name 23-K020244-022-308-F08C-8409
GenBank Accession LN496374 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K020244-022-308-F08C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTAACAGTTTAGCTC
AGTGGTTGTGAAGATAACTGCCATTTGTCCAATTAGTCTTCTGAAACGAGTGAGCGATCT
GCTGCGGTGGGAATACAAAAGTCCGAACTTCAAACTCTCATGGAAGCTCAAATCGTTTCC
GGTTTTCTCCGAATCGAGTCCTCTCTACCACACAAACTCAGAACCGGAACCGTTAACCGC
GGAAGAAGAAAGGGAGCTCGAAGCAGCTCATGGAAGGATTCAAGAAATCTGTAGGAAATG
CCAAGAGTCCAATGTACCATTGTTGATTGATGCGGAAGACACAATCCTCCAACCCGCGAT
CGATTACATGGCTTATTCATCGGCGATCATGTTCAATGCTGACAAAGACCGACCAATCGT
TTACAACACGATTCAGGCGTACTTGAGAGACGCCGGTGAGAGACTGCATTTGGCAGTACA
AAATGCTGAGAAAGAGAATGTTCCTATGGGGTTCAAGTTGGTGAGAGGGGCTTACATGTC
TAGCGAAGCTAGCTTGGCGGATTCCCTGGGTTGCAAGTCGCCAGTCCACGACACAATTCA
GGATACTCACTCTTGTTACAATGATTGTATGACATTCCTGATGGAGAAAGCATCAAACGG
TTCTGGTTTCGGTGTCGTTCTCGCAACACATAACGCTGATTCGGGTAAGC
   
Sequence Name 24-K020244-022-308-F08C-MR52
GenBank Accession LN496815 [GenBank]
Sequencing primer gene specific primer, MR52: 5'-ACAATGTGATTTAATGGCTTACCC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K020244-022-308-F08C-MR52
GAATCACGTTATGTGTTGCGAGAACGACACCGAAACCAGAACCGTTTGATGCTTTCTCCA
TCAGGAATGTCATACAATCATTGTAACAAGAGTGAGTATCCTGAATTGTGTCGTGGACTG
GCGACTTGCAACCCAGGGAATCCGCCAAGCTAGCTTCGCTAGACATGTAAGCCCCTCTCA
CCAACTTGAACCCCATAGGAACATTCTCTTTCTCAGCATTTTGTACTGCCAAATGCAGTC
TCTCACCGGCGTCTCTCAAGTACGCCTGAATCGTGTTGTAAACGATTGGTCGGTCTTTGT
CAGCATTGAACATGATCGCCGATGAATAAGCCATGTAATCGATCGCGGGTTGGAGGATTG
TGTCTTCCGCATCAATCAACAATGGTACATTGGACTCTTGGCATTTCCTACAGATTTCTT
GAATCCTTCCATGAGCTGCTTCGAGCTCCCTTTCTTCTTCCGCGGTTAACGGTTCCGGTT
CTGAGTTTGTGTGGTAGAGAGGACTCGATTCGGAGAAAACCGGAAACGATTTGAGCTTCC
ATGAGAGTTTGAAGTTCGGACTTTTGTATTCCCACCGCAGCAGATCGCTCACTCGTTTCA
GAAGACTAATTGGACAAATGGCAGTTATCTTCACAACCACTGAGCTAAACTGTTAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCACCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTTACCATTTTT

Last Updated on 06.12.2018 16:11