Genotyping and primer details


Genotyping and primer details for 308F08 - At3g30775
Line ID 308F08
Locus At3g30775 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:12450004 of line 308F08 Chr3:12450004
   
Insertion allele amplicon 8474 - MR52
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MR52: 5'-ACAATGTGATTTAATGGCTTACCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:12449322
   
wt amplicon (over InsPos) 06gy - MR52
wt amplicon start Chr3:12449322
wt amplicon end Chr3:12450317
wt amplicon size 996 bp
Locus specific primer MR52: 5'-ACAATGTGATTTAATGGCTTACCC-3'
2nd locus specific primer 06gy: 5'-CGCGTTATACAAGTACGTACCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11