Sequences from confirmation amplicon


Confirmation sequences for line 308G07 and hit At1g77460 are shown.
Line ID 308G07
Locus At1g77460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29108879
Primers used for PCR 34by - 8474, show primer details
   
Sequence Name 25-K108223-0022-308-G07C-34by
GenBank Accession LN497476 [GenBank]
Sequencing primer gene specific primer, 34by: 5'-GTAAATCTATCCCGCTGCTTGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K108223-0022-308-G07C-34by
CATTCGATGGCCTGGAGCCCTCAGTTTGCTGTCCAATCCTGATTCGCATTGCTGATGGAA
GTGACACAAATAAATTACTTATGGCTGAAGCTGGAGCTGTTGAAGCTTTAACAAAGTACC
TTTCTCTAAGTCCTCAAGATTCAACTGAGTACGCCATATCTGAATTGCTTAGGGTATTAT
TTAGCAACCACGAGCTTAGACAAAACGAAATGGCACTAAGTTCCTTGAATCAGCTTATCG
CAGTACTGCGGCTAGGTTCACGAAGTGCAAGATACAGCGCAGCTGGGGCTCTGAATGAAC
TATTTGATGCTGAAAACATCAGAAATTCTGAAATCGCCTGCCAGGCTGTTCAACCACTTA
TGGACATACTCGGTTCTGTATCAGAAAGTGAGCAAGAGGTAGCTCTCTCTGCATTGATCA
AACTAAGCTCAGGAAATACTTCGAATACAGCTCTTCTGATTGATGTGGAAGGAAGTCTGC
TGGCTTCGTTTCCCGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 09-K108222-0022-308-G07C-8409
GenBank Accession LN490213 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K108222-0022-308-G07C-8409
TTGAATATATCCGGGAAACGAAGCCAGCAGACTTCCTTCCACATCAATCAGAAGAGCTGT
ATTCGAAGTATTTCCTGAGCTTAGTTTGATCAATGCAGAGAGAGCTACCTCTTGCTCACT
TTCTGATACAGAACCGAGTATGTCCATAAGTGGTTGAACAGCCTGGCAGGCGATTTCAGA
ATTTCTGATGTTTTCAGCATCAAATAGTTCATTCAGAGCCCCAGCTGCGCTGTATCTTGC
ACTTCGTGAACCTAGCCGCAGTACTGCGATAAGCTGATTCAAGGAACTTAGTGCCATTTC
GTTTTGTCTAAGCTCGTGGTTGCTAAATAATACCCTAAGCAATTCAGATATGGCGTACTC
AGTTGAATCTTGAGGACTTAGAGAAAGGTACTTTGTTAAAGCTTCAACAGCTCCAGCTTC
AGCCATAAGTAATTTATTTGTGTCACTTCCATCAGCAATGCGAATCAGGATTTGGACAGC
AAACTGAGGGGCTCCAGGCCTATCTGGAATTGGCCTTAAGAGATCTACAAGCAGCGGGAT
AGATTTA

Last Updated on 06.12.2018 16:11