Sequences from confirmation amplicon


Confirmation sequences for line 310B09 and hit At3g17800 are shown.
Line ID 310B09
Locus At3g17800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6091406
Primers used for PCR AB60 - 8474, show primer details
   
Sequence Name 57-K105307-0022-310-B09C-AB60
GenBank Accession LN509415 [GenBank]
Sequencing primer gene specific primer, AB60: 5'-TCAATCCCAAGCTACTGAAATTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K105307-0022-310-B09C-AB60
GTCTGCTCGTTCCTCACTCGGACGCAGAGATCGTCTTCTACGATGTCTTTGGTGAGATTT
TGCACAAATGGGTCACTTCTAAACAACTTCTTCATTCCTCTGTTTGAAGATAAAAAGCCT
TAATAAATTTGGCTTCCTTTGTAATTTTGTAACATACATTTAAGATTCTTAAGTCCTATT
GCAGGCTAGGTAGGCTTCCTGCTTAGTTTAGCATCCCTATCATTTTTAGCAATAGCTTAT
TCCATCACAATAACATATATAATGTACGATCTTTTCCTCAAATTTTTGATACAAGTGTAA
TGATACCCTTAATGGGTATCTTCTTAATGCTACATAAGTTAGTCAACTGCATTACAGACC
ATGTTTTTAGTTCAATACAAAGTGGTACCTCGCATCTACATGGCTCTCAACATCCCAAAG
GAACGACCCGAAAGTAACTGCCTCCAAGACGAGCCTCTTCATTCCACCGAAGCTGATCTA
CATGGAAATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCC
   
Sequence Name 41-K105308-0022-310-B09C-8409
GenBank Accession LN504077 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K105308-0022-310-B09C-8409
GCCATTTACAATTGATTTCATGTAGATCAGCTTCGGTGGAATGAAGAGGCTCGTCTTGGA
GGCAGTTACTTTCGGGTCGTTCCTTTGGGATGTTGAGAGCCATGTAGATGCGAGGTACCA
CTTTGTATTGAACTAAAAACATGGTCTGTAATGCAGTTGACTAACTTATGTAGCATTAAG
AAGATACCCATTAAGGGTATCATTACACTTGTATCAAAAATTTGAGGAAAAGATCGTACA
TTATATATGTTATTGTGATGGAATAAGCTATTGCTAAAAATGATAGGGATGCTAAACTAA
GCAGGAAGCCTACCTAGCCTGCAATAGGACTTAAGAATCTTAAATGTATGTTACAAAATT
ACAAAGGAAGCCAAATTTATTAAGGCTTTTTATCTTCAAACAGAGGAATGAAGAAGTTGT
TTAGAAGTGACCCATTTGTGCAAAATCTCACCAAAGACATCGTAGAAGACGATCTCTGCG
TCCGAGTGAGTGAACCGAGCAGACATGAATCCTTGACCATCATAATAGAATTTCAGTAGC
TGGGGGATTG
Primers used for PCR 34ah - 8474, show primer details
   
Sequence Name 46-K119268-0022-310-B09C-34ah
GenBank Accession LN506024 [GenBank]
Sequencing primer gene specific primer, 34ah: 5'-CTGAACTCTGTTGCACAGATAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K119268-0022-310-B09C-34ah
TTCTGTGATGTTGGATATTTCTTGAAGAGGGTGGACCAAAGGTTTCAGCTTGAGAAGACG
ATGAAGATTCTTCCGGGTGGGTCGGATGAAAGTAAAACAAGCGTTGAGCAAGCCGAGGGG
ACGGCAACATATCAGGCGGCTGTATCGTCTCACCCAGAGGTTGGTGCATTTGCGGGCGGA
GTTAGTGCTAAGGGCTTCGGCAGCGAGATTAAACCATCCCGATTAAGGTCATATGTGATG
TCATTTGATGCGGAGACGCTGCAGAGATACGCCACTATAAGATCAAGAGAAGCAGTTGGA
ATCATCGAGAAGCACACAGAGGCATTGTTTGGTAAGCCTGAGATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGACACATTATTAT
   
Sequence Name 22-K119267-0022-310-B09C-8409
GenBank Accession LN496337 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K119267-0022-310-B09C-8409
CATTTACAATCTCAGGCTTACCAAACAATGCCTCTGTGTGCTTCTCGATGATTCCAACTG
CTTCTCTTGATCTTATAGTGGCGTATCTCTGCAGCGTCTCCGCATCAAATGACATCACAT
ATGACCTTAATCGGGATGGTTTAATCTCGCTGCCGAAGCCCTTAGCACTAACTCCGCCCG
CAAATGCACCAACCTCTGGGTGAGACGATACAGCCGCCTGATATGTTGCCGTCCCCTCGG
CTTGCTCAACGCTTGTTTTACTTTCATCCGACCCACCCGGAAGAATCTTCATCGTCTTCT
CAAGCTGAAACCTTTGGTCCACCCTCTTCAAGAAATATCCATACATCACAGAAGCAGCGT
AAACTTGGCCAACTCTGAGCTTGCTTATCTGTGCAACAGAGTTCAGA

Last Updated on 06.12.2018 16:11