Sequences from confirmation amplicon


Confirmation sequences for line 310D02 and hit At3g16360 are shown.
Line ID 310D02
Locus At3g16360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5555046
Primers used for PCR BV16 - 8474, show primer details
   
Sequence Name 83-K105916w-0022-310-D02C-BV16
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, BV16: 5'-GATGATGCAAACCCTAATTTTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K105916w-0022-310-D02C-BV16
CTCGGTTATCAATAACATTGACCAAGCTTTGTGAGTATTATTTTGTTTTCTATTATATAT
ATATTTTTTTTTTTTCAGTTTTCTAACCATTTTTACAATGCAGGGAAAAAGGATCATTTG
ATTTCAACCGGCGGGATAGTTCCATGCATCATTTTAAGGGAACCACCCCAAGGTAATTCC
CCCCCTAATTTTTTGACTATCCTAATTACCCCTTCAAAAAATAATTTTACTTAAAAAAAT
GCAAAACCTTAAATGTTTATAAAAAAAAAAAATGTAACCCAAAAGTCCATTAAAATTACT
TCCTTTCCCATTCAGGATTCAATGACAAAATCTCCTTTACCCAAAAAAAATTAAATTTTA
TGACCGTTAGTTATCCGGACAAAGGTTGCATTTCTTTTTTTTGAACCATTGGGGCAAGTA
AAGTGAAACCTAAATCACCATTGCATTACTGAAACAAAAATGTAAAGAGTAATCTCCAAG
GGCTTCATAGGGCAGGAAATATCCAATTGAAAAGGGCTCCATGCCCGGAAAATCTCCTGG
GATCACCAATGAGTATAAGGGTCAATATGGAAAAAAAAAAAAAGTATTTCCCATTTTTTT
TTCATTTCAAAAATGT
   
Sequence Name 84-K105917w-0022-310-D02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K105917w-0022-310-D02C-8409
GCCATTTACAATTGAATATATCCTGCACTATGAAGCCATTGCAGATTACTCTTTACATTT
TTGTTTCAGTATTGCATTGCTGATTCAGCTTTCACTTTACTTGCCCCAATGCTACAAAAA
AAAGAAATGCAACATTTGTCAGTATAACTAACGATCATAAAATCTAATTATTTCTGGTTA
AAGTAGATTCTGTCATTGAATCCTGAATGAGAATGGAAGTAATTTTAATGTACTTTTGCG
TTACATTCTTTTATCTTATAAACATTTAAGTATTCGCATTTCTTTAAGTAAAATTATTTC
TTGAAGGCGTAATTAGAATAGTCAAAAACTAAGGGGGGAAATTACCTCGTGCTGCTTCCC
TTAAACTGATGCATGTAACTATCCAGCCGATTGAAATCAAATGATCCTCTTTCCCTGCAT
TGTAAAAATGATTAGAAAACTGAAAAAAAAAAAATTTTTTTTTAATAAAAAACAAAATAA
TCCTCACAAAGCTGGGTCAATGTTTTTGATTAACCGAGCTAAATCTTTAAAGTAAAAGGC
GAAAACTTCTTCAACAA

Last Updated on 06.12.2018 16:11