Sequences from confirmation amplicon


Confirmation sequences for line 310E01 and hit At4g33410 are shown.
Line ID 310E01
Locus At4g33410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16082693
Primers used for PCR 17ql - 8474, show primer details
   
Sequence Name 68-K115459-0022-310-E01C-17ql
GenBank Accession LN512072 [GenBank]
Sequencing primer gene specific primer, 17ql: 5'-CTCCATTTGTATTGCGTTTGTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K115459-0022-310-E01C-17ql
CTCCTCGTGTGTCTCTTTGTATATGACATATTCTGGGTTTACTTCTCTGAGAGATTCTTT
GGTGCTAACGTTATGGTGGCCGTGGCAACCCAGCAAGCATCAAACCCAGTTCACACAGTG
GCTAACAGTTTGAATCTTCCTGGACTGCAATTGATCACAAAGAAACTGGAACTACCTGTT
AAAATTGTGTTTCCTCGGAACCTATTGGGCGGTGTGGTGCCCGGTGTGAGTGCCTCAGAT
TTCATGATGCTTGGTCTTGGTGACATGGTACAGACTAATCCCTTCTTTGATTTTAAGATT
GGTATGTCGTTATATGTGTACTAACTGTAAGAAATGTTCTCTCTCAGACTATAGTGACGA
GGAGATAAAAAAAACATGTTGTATTGATTAACTGTTTTGTTGTTCCAGGCTATCCCTGCA
ATGCTTTTGGCTTTGGTCCTCTGCTTTGACCATCGAAAAACTAGAGATGTGGTGAATATT
TTTGATCTAAAATCTTCCAAGGGACACAAATACATTTGGTATGTCATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 19-K115458-0022-310-E01C-8409
GenBank Accession LN494892 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K115458-0022-310-E01C-8409
TTGAATATGACATACCAAATGTATTTGTGTCCCTTGGAAGATTTTAGATCAAAAATATTC
ACCACATCTCTAGTTTTTCGATGGTCAAAGCAGAGGACCAAAGCCAAAAGCATTGCAGGG
ATAGCCTGGAACAACAAAACAGTTAATCAATACAACATGTTTTTTTTATCTCCTCGTCAC
TATAGTCTGAGAGAGAACATTTCTTACAGTTAGTACACATATAACGACATACCAATCTTA
AAATCAAAGAAGGGATTAGTCTGTACCATGTCACCAAGACCAAGCATCATGAAATCTGAG
GCACTCACACCGGGCACCACACCGCCCAATAGGTTCCGAGGAAACACAATTTTAACAGGT
AGTTCCAGTTTCTTTGTGATCAATTGCAGTCCAGGAAGATTCAAACTGTTAGCCACTGTG
TGAACTGGGTTTGATGCTTGCTGGGTTGCCACGGCCACCATAACGTTAGCACCAAAGAAT
CTCTCAGAGAAGAAAACCCAGAATATGTCATATACAAAGAGACACACGAGGAGCATTGCA
CAGATTTTGATATTAGGAAGACGAACATGGCTAACAAACGCATAAAAAATGGA

Last Updated on 06.12.2018 16:11