Sequences from confirmation amplicon


Confirmation sequences for line 310H05 and hit At1g07230 are shown.
Line ID 310H05
Locus At1g07230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2221007
Primers used for PCR 20qf - 8474, show primer details
   
Sequence Name 33-K115400-0022-310-H05C-20qf
GenBank Accession LN501122 [GenBank]
Sequencing primer gene specific primer, 20qf: 5'-GATAAGACTGGTGTGGGATTGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K115400-0022-310-H05C-20qf
TCACACCCATTCCAATTTGAGCATTCTTCTATCCCTGCAACGGTGAAGAAACTCTTCAAC
CTAAAGTCTCACTTCCTTACAAAGAGAGATGCATGGGCTGGTACATTTGAGAAATACTTC
CGTATCCGAGATTCTCCTCGCCAAGATTGTCCAGGTCTGTTTCATTCTCTACCTCATTTC
CCACTCCATTACATGCTCTCTGCTTTTTTGGCTCTAAATCCTTTGCATTATGTAATCACC
AAATAAGGGCCATTACAAATATGTAAAGTAATGTTTCATTAAGTATCTCACCGCATTTGT
AGACAGTTACAGTTGGTTGAACTTGAGTCAACATAGTGTTTGAAAGATAGTCTTGATCAA
TATGAGTATGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATA
   
Sequence Name 09-K115399-0022-310-H05C-8409
GenBank Accession LN490319 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K115399-0022-310-H05C-8409
TCAGACTATCTTTCAACACTATGTTGACTCAAGTTCAACCAACTGTAACTGTCTACAAAT
GCGGTGAGATACTTAATGAAACATTACTTTACATATTTGTAATGGCCCTTATTTGGTGAT
TACATAATGCAAAGGATTTAGAGCCAAAAAAGCAGAGAGCATGTAATGGAGTGGGAAATG
AGGTAGAGAATGAAACAGACCTGGACAATCTTGGCGAGGAGAATCTCGGATACGGAAGTA
TTTCTCAAATGTACCAGCCCATGCATCTCTCTTTGTAAGGAAGTGAGACTTTAGGTTGAA
GAGTTTCTTCACCGTTGCAGGGATAGAAGAATGCTCAAATTGTGAATGTGGTGTTGGACC
TTCAGGTTCATGTATCACTGCAACGAAATCAATCCCACACCAGTCTTAT

Last Updated on 06.12.2018 16:11