Sequences from confirmation amplicon


Confirmation sequences for line 311F09 and hit At2g32560 are shown.
Line ID 311F09
Locus At2g32560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13825525
Primers used for PCR BV17 - 8474, show primer details
   
Sequence Name 14-K105950-0022-311-F09C-BV17
GenBank Accession LN492511 [GenBank]
Sequencing primer gene specific primer, BV17: 5'-TTGATTCTCCTCATCATCAAACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K105950-0022-311-F09C-BV17
CTCTCTGATCCGATCTCTTTATCCGTTTTCCGAGAGGATAAACAAAGGAGGGGATATGCA
TCTTCTCTGCCACTTGATTCCAGCATGAGCTGCTACCTCTCCCTTGAAACAGGTCGTTTT
TGGTTCCCAGCTCAAGTTTACAACCGTGAGGTAATCATTTCATAAACATAGTTGCTCCAT
TTTTGCTTTATGGATTTGATTAAAGTACTAAGAGGCTTTTTTTTGGTGAAAGAGCAGAAT
GGACATGTAGGGTTCATGTTGTCATGCTATGATGCGGAGCTCAGCTATGATACTCAGCAA
TGAGTATATATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCG
   
Sequence Name 30-K105951-0022-311-F09C-8409
GenBank Accession LN499724 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K105951-0022-311-F09C-8409
ACATGAACCCTACATGTCCATTCTGCTCTTTCCCAAAAAAAAGCCTCTTAGTACTTTAAT
CAAATCCATAAAGCAAAAATGGAGCAACTATGTTTATGAAATGATTACCTCACGGCTTGT
AAACTTGATGCTGGGAACCAAAAACGACCTGTTTCAAGGGAGAGGTAGCAGCTCATGCTG
GAATCAAGTGGCAGAGAAGATGCATATCCCCTCCTTTGTTTATCCTCTCGGAAAACGGAT
GAAAGAGATCGGATCAGAGAGATGATTTTGGCAAAACCAACATTCCCAGTTTGATGATGA
GGAGAATCAAACTGGG

Last Updated on 06.12.2018 16:11