Sequences from confirmation amplicon


Confirmation sequences for line 311H11 and hit At1g04280 are shown.
Line ID 311H11
Locus At1g04280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1145402
Primers used for PCR 22cp - 8474, show primer details
   
Sequence Name 25-K108475-0022-311-H11C-22cp
GenBank Accession LN497478 [GenBank]
Sequencing primer gene specific primer, 22cp: 5'-CATCTCAAGAATGAACATGGCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K108475-0022-311-H11C-22cp
AAAGGATGCTAACATTTCAGTAAAGAAGATAGAAAAATAATCAAGATCGACCAAAGGATT
AGAATAACATAAAAATAAATTAGAGTATTTCTTAGGCAGACGGAAAAAAAGAAGAGTTAC
TATTTCTTGGGTTAATATGCCAAAAAAAATAAAAATATGTATAGGTAAGCATTAATGTTT
TGGTGATAAGAGATTTCAAAATGGGGTATGTACACGTACTCTTTATCCATAAAATCATAA
ATGCTAGAAGACCTTTCCACAACAACAAAAAATGAGCCCTTTGTAATTATTTTATCCAAA
CATAATTTTGGAACAATTTTGTGTAATCATATATATGTTAAAAATGCAGCAAGGCAGCTC
GGATTTGAGGATCCAAATGAATACCCACAACTATGCAAATTAGCAAATGGATATTTGTTG
AAAACAAAAGGGTATGATGAGAATGTGGATGAGTATTTGGAGAATGAAGCTGAGCGTGAT
TCTCTCTATGTTCATCTCCTTGAAGAATTTGATAGATGCATTCTCACTTATTTTTCTTTC
AATTGGACCCAATCTTCCAACCTCATCTCTCAGGTACTTTGATTTCAATCAAATTCAGTA
TATTTTTATTTGGTAGTCTGATAGGTGAAGTAGGCTATTTACTACAACCATTAGCGATCT
CCAATAATGTGTATGAATAATAAATATTATCCTATAACACAAAATGACAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAAAAGTTATT
   
Sequence Name 09-K108474-0022-311-H11C-8409
GenBank Accession LN490215 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K108474-0022-311-H11C-8409
ATTATTGGAGATCGCTAATGGTTGTAGTAAATAGCCTACTTCACCTATCAGACTACCAAA
TAAAAATATACTGAATTTGATTGAAATCAAAGTACCTGAGAGATGAGGTTGGAAGATTGG
GTCCAATTGAAAGAAAAATAAGTGAGAATGCATCTATCAAATTCTTCAAGGAGATGAACA
TAGAGAGAATCACGCTCAGCTTCATTCTCCAAATACTCATCCACATTCTCATCATACCCT
TTTGTTTTCAACAAATATCCATTTGCTAATTTGCATAGTTGTGGGTATTCATTTGGATCC
TCAAATCCGAGCTGCCTTGCTGCATTTTTAACATATATATGATTACACAAAATTGTTCCA
AAATTATGTTTGGATAAAATAATTACAAAGGGCTCATTTTTTGTTGTTGTGGAAAGGTCT
TCTAGCATTTATGATTTTATGGATAAAGAGTACGTGTACATACCCCATTTTGAAATCTCT
TATCACCAAAACATTAATGCTTACCTATACATATTTTTATTTTTTTTGGCATATTAACCC
AAGAAATAGTAACTCTTCTTTTTTTCCGTCTGCCTAAGAAATACTCTAATTTATTTTTAT
GTTATTCTAATCCTTTGGTCGATCTTGATTATTTTTCTATCTTCTTTACTTGAAATTGTT
AGCAATCCTTTTTTTGTTAATTAAAAGTATTAGCCATGTTCATTCTTGAGATG

Last Updated on 06.12.2018 16:11