Sequences from confirmation amplicon


Confirmation sequences for line 312D02 and hit At4g29010 are shown.
Line ID 312D02
Locus At4g29010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14297407
Primers used for PCR 10ve - 8474, show primer details
   
Sequence Name 62-K118152-0022-312-D02C-10ve
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 10ve: 5'-CCCTTGTTTACTGTGTCGGATTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K118152-0022-312-D02C-10ve
ATTTGAAGTAATATATAATAAATCCTTAATTTGATTATAAAAAAAAAGATGTAAATTTCT
TCATATTTTTCTCTCTGGCTAGAACAAATCATAACTTTATGAAACTGACGTCACTACAAA
CATTTATTAAGAGCAGAAAATTGTTTATTTTAAGAGTGTTAACAACAACCACACCTTAAT
AGCATTTTAACTTTGAACTTTGAAACAACAAGAGAGACTGATTTATTTTCATTTAGTGAC
TAACACCTTCAGCCTCTCCTTCTGCATATACTTAATTAAACGCTTCACAATTTGGACCTC
GACGATTTCGATTCACTCTGCAGCCAAAACCAGAATTTTCATCATGAATAAATCGAAACA
AAACTACATGGAGATCTCTATGATGGAATTGACCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCACACGGTTATT
   
Sequence Name 14-K118151-0022-312-D02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K118151-0022-312-D02C-8409
CATTTCATTGAATTATCCTGGGTCAATTCCATCATAGAGATCTCCATGTAGTTTTGTTTC
GATTTATTCATGATGAAAATTCTGGTTTTGGCTGCAGAGTGAATCGAAATCGTCGAGGTC
CAAATTGTGAAGCGTTTAATTAAGTATATGCAGAAGGAGAGGCTGAAGGTGTTAGTCACT
AAATGAAAATAAATCAGTCTCTCTTGTTGTTTCAAAGTTCAAAGTTAAAATGCTATTAAG
GTGTGGTTGTTGTTAACACTCTTAAAATAAACAATTTTCTGCTCTTAATAAATGTTTGTA
GTGACGTCAGTTTCATAAAGTTATGATTTGTTCTAGCCAGAGAGAAAAATATGAAGAAAT
TTACATCTTTTTTTTTATAATCAAATTAAGGATTTATTATATATTACTTTCAAATCCTAC
AGAAGCTGATTGAGAAACATCTCAGATCATAATCCGACACAGTAAACAAGGG
Primers used for PCR 73xr - 8474, show primer details
   
Sequence Name 25-K119356-0022-312-D02C-73xr
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 73xr: 5'-GATATATGCCAAGGTCTATGGTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K119356-0022-312-D02C-73xr
TCTTAGAGCGGGCGAAAGGTATGTCATTGCTCTTACCTTTTGACATTCCTAATGTGTCCA
CTTTTAACATTTCTGGCTTCAGGAAAAATCAACGGAAGAGGATACTATATATACGAAAAG
GGAAGCAAACCAAAACCTGATCCATCAGTGCTTTCAATTGTTGAGAAATCAAGGAAACTG
ACCAATATCATGCCTGGTGGGAAGGTAAAAAAAGAGGAGGTCTTATGAAATATATCAGTA
CTACTATTGGTATTTAAAAAAATGAAATGCACTATTGTCTGTTCTTCTTTTGCAGCCTAT
ATCAGTAACTGATAAAGAAATTGTAGAGATGATCTTATTCCCTGTTGTCAACGAGGCATG
CCGCGTCCTAGATGAAGGAGTTGTGATCCGAGCCTCAGACTTGGACATTGCGTCTGTCCT
TGGAATGAGTTTTCCTTCTTACCGGTGAGGATTCTAAGTCATAATCCCCATAAAGCAAAA
GGGAGCATTTTGTGAAATTCTGGAAATGCATTACCTCTGCTCAAATCTTATGCATATGAT
TTTTTCACATCTTTGAGTTTCTTCTGGTCATTTACAAACGTTCACAATATCCTCAAAACT
TTTTCCTTTCTTTTGTTAGTCACTAAGATTTTTGGTGTATGATCATTTCTTATGTTAAGC
AATTTTTTTTACCTGTTGCAGGGGAGGAATTGTTTTCTGGGCAGACACTGTTGGACCAAA
GTACATATATGAGAGGCTCAAGAAATTGTCGGAGACTTATGGCAGCTTTTTCAAACCATC
GAGGTATCTGGAGGAAAGAGCAATGAATGGAATGCTTTTGGTAAAGTTTCTCAACTTTCT
TTACATTTTATGATGTGGCAAGAAAACACAGTACCAACCCAGTTCTCTTATTAACAAATC
GAT
   
Sequence Name 05-K119355-0022-312-D02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K119355-0022-312-D02C-8409
ATCATCATACTCATACGCTTGGAAACATTTCCATCATAGAGATCTAGTTATTATCATAAA
TTGTTTACTCAAAACTTCATTTTTACTAGCCAAACTACAAAACAATTCTACATAGTTCAT
GCCATACTTGAACCCAAAATCTAATGTTAGCAGGTCCCTTGCAGCAGATGAATCAACCTA
AATTCTATTCTCATATCGTTTATCGATTTGTTAATAAGAGAACTTGGTTGGTACTGTGTT
TTCTTGCCACATCATAAAATGTAAAAGAAAGTTGAGAAACTTTACCAAAAGCATTCCATT
CATTGCTCTTTCCTCCAGATACCTCGATGGTTTGAAAAAGCTGCCATAAGTCTCCGACAA
TTTCTTGAGCCTCTCATATATGTACTTTGGTCCAACAGTGTCTGCCCAGAAAACAATTCC
TCCCCTGCAACAGGTAAAAAAAATTGCTTAACATAAGAAATGATCATACACCAAAAATCT
TAGTGACTAACAAAAGAAAGGAAAAAGTTTTGAGGATATTGTGAACGTTTGTAAATGACC
AGAAGAAACTCAAAGATGTGAAAAAATCATATGCATAAGATTTGAGCAGAGGTAATGCAT
TTCCAGAATTTCACAAAATGCTCCCTTTTGCTTTATGGGGATTATGACTTAGAATCCTCA
CCGGTAAGAAGGAAAACTCATTCCAAGGACAGACGCAATGTCCAAGTCTGAGGCTCGGAT
CACAACTCCTTCATCTAGGACGCGGCATGCCTCGTTGACAACAGGGAATAAGATCATCTC
TACAATTTCTTTATCAGTTACTGATATAGGCTGCAAAAGAAGAACAGAACATAGTGCATT
TCATTTTTTTAAATACCAATAGTAGTACTGATATATTTCATAAGACCTCCTCTTTTTTTA
CCTTCCCACAGGCATGAAATTGGTCAGTTTCCTGATTTCT

Last Updated on 06.12.2018 16:11