Genotyping and primer details


Genotyping and primer details for 312D02 - At4g29010
Line ID 312D02
Locus At4g29010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14297407 of line 312D02 Chr4:14297407
   
Insertion allele amplicon 8474 - 10ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10ve: 5'-CCCTTGTTTACTGTGTCGGATTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14296970
   
Insertion allele amplicon 8474 - 73xr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 73xr: 5'-GATATATGCCAAGGTCTATGGTGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14298560
   
wt amplicon (over InsPos) 73xr - 10ve
wt amplicon start Chr4:14296970
wt amplicon end Chr4:14298560
wt amplicon size 1591 bp
Locus specific primer 10ve: 5'-CCCTTGTTTACTGTGTCGGATTAT-3'
2nd locus specific primer 73xr: 5'-GATATATGCCAAGGTCTATGGTGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11