Sequences from confirmation amplicon


Confirmation sequences for line 312E07 and hit At5g65340 are shown.
Line ID 312E07
Locus At5g65340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26114117
Primers used for PCR FV61 - 8474, show primer details
   
Sequence Name 33-K100896-0022-312-E07C-8409
GenBank Accession LN500919 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K100896-0022-312-E07C-8409
CCCGCGAAATGCAAGGAGGCGTGCTCAGGATCGCTCTCGAATCAAACAATAACGACGGAT
ATGATTCACATGAAGATTCTTCCTCTTCACTTCTTACAACACCTTTATGGAACATGTATT
GCAACGGTCGGAAAGTTGGATTCGCCATCAAACGAGAGCCTTCGAAGTCTGAGCTAGCTG
CGTTGAAGGTGCTTACTCCGGTGGCTGAAGGAGCCGGAGTTGTTAACGGAGAAGAGATAA
ACCGAGAGAAAAGTGATCATATGATGTACTTAAGAGCGAGTTTCAAACGAGTTTTTGGTT
CGTTTGATTCGGAATCTTTCCATCTCGTTGACCCACGTGGGATTATCGGTCAAGAACTAA
GTATCTTCTTTTCTAGATCGTCAAAAAGATGATCTTGGATCGTTTTGTGT
   
Sequence Name 33-K100904-0022-312-E07C-FV61
GenBank Accession LN500920 [GenBank]
Sequencing primer gene specific primer, FV61: 5'-TCCTTGTGCTAGAACACACAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K100904-0022-312-E07C-FV61
AAAGAAGATACTTAGTTCTTGACCGATAATCCCACGTGGGTCAACGAGATGGAAAGATTC
CGAATCAAACGAACCAAAAACTCGTTTGAAACTCGCTCTTAAGTACATCATATGATCACT
TTTCTCTCGGTTTATCTCTTCTCCGTTAACAACTCCGGCTCCTTCAGCCACCGGAGTAAG
CACCTTCAACGCAGCTAGCTCAGACTTCGAAGGCTCTCGTTTGATGGCGAATCCAACTTT
CCGACCGTTGCAATACATGTTCCATAAAGGTGTTGTAAGAAGTGAAGAGGAAGAATCTTC
ATGTGAATCATATCCGTCGTTATTGTTTGATTCGAGAGCGATCCTGAGCACGCCTCCTTG
CATTTCGCGGGCTAATACCTCGGTAGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGA
Primers used for PCR FV62 - 8474, show primer details
   
Sequence Name 34-K100896-0022-312-E07C-OriL
GenBank Accession LN501338 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K100896-0022-312-E07C-OriL
GCCATTTACAATTGAATATTGGGTTTAGGGTTTTGGAGTTTGTTGCTTGGATGCAGAAGT
TTACTTTGCCACGACGGAAACCGAATATGGTTCCGGTAAAAGTGGAGTTTGTGGCGGTTG
AGGAGGCGGAAGAGGTTCTTGGTTTAATTTGGCGGTGAGATAATTTGTCTTCTTGACTAT
CATTTGGATCGACGATTGTGCAGTAACAAGATGGGATGAGAAGTTGGGCGATGGTGCGAA
GGAGCCGCCATGAACGAACTTGTTTATGGCAGTCTACGGCTACCGTTGCGCTTGTGGTGG
TTGAAGCTAGGCGGCGACGGTAAGGGTTTGTGGGGAAGGCGGACATGGTTTTTTTTTGGG
AGTTGTACGAATGGGATGAGAATTTTATGTGTGTTAGGGTTTAGGTGGCATGGATGTATA
TATAGAAAATGGGCATGACAATAAAATGATATGAACTTGTGTTGTTGTTTTCTCCTTACT
TCAAATTTTTTTATACAAATCAAAAGCAACCTCAATTGATTCTAATATATTATAAATTAT
ACTATATCTCAAATGATTTATGTGAAATAAATTTCATTATTACATAATAGAAAA
   
Sequence Name 34-K100904-0022-312-E07C-FV62
GenBank Accession LN501339 [GenBank]
Sequencing primer gene specific primer, FV62: 5'-TCCGCGTACAATAATCCTTTTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K100904-0022-312-E07C-FV62
AACAGTTTTAGTTTTAAATGGATTGATTTGTTTTCTATTATGTAATAATTAAATTTTATT
CACATAAATCATTTTAGATATAGTATAATTTATAATATTTTAGAATCAATTGAGGTTGCT
TTTGATTTGTATAAAAAAATTTGAAGTAAGGAGAAAACAACAACACAAGTTCATATCATT
TTATTGTCATGCCCATTTTCTATATATACATCCATGCCACCTAAACCCTAACACACATAA
AATTCTCATCCCATTCGTACAACTCCCAAAAAAAAACCATGTCCGCCTTCCCCACAAACC
CTTACCGTCGCCGCCTAGCTTCAACCACCACAAGCGCAACGGTAGCCGTAGACTGCCATA
AACAAGTTCGTTCATGGCGGCTCCTTCGCACCATCGCCCAACTTCTCATCCCATCTGGTT
ACTGCACAATCGTCGATCCCAATGATAGTCAAGAAGAACAATTATCTCACCGCCCAATTA
AACCAAGAACCTCTTCCGCCTCCTCAACCGCCACAAAATCCACTTTTACCGGAACCATAT
TCCGTTTCCGTCGTGGCAAAGTAAACTTCTGCATCCCAGCAACAAACTCCCAAAC

Last Updated on 06.12.2018 16:11