Sequences from confirmation amplicon


Confirmation sequences for line 312H09 and hit At2g45160 are shown.
Line ID 312H09
Locus At2g45160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18618138
Primers used for PCR 12lu - 8474, show primer details
   
Sequence Name 49-K114086-0022-312-H09C-12lu
GenBank Accession LN506917 [GenBank]
Sequencing primer gene specific primer, 12lu: 5'-AAATTAACAGTCTTTGCTCCTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K114086-0022-312-H09C-12lu
CAGAGGAAATCTCAAAACATTCGCCGGAGAAGTCAAGATTCCTTTCGAAATCGAGTTACT
AAGCGTAGAGCTTCTTCTAAACCCAGCTTATTGGCCACTCTCTCTACGTTCATCGGAAAA
AGAAGCAATCGCAGTGAATCTTCCAGTAAACTCCGTCGCCTCCGGTTACCTTCCGTTAAT
CCTCCGATTCCTTAAACAACTATCACCAAACATCGTCGTTTGCTCAGACAGAGGATGTGA
CCGTAACGACGCGCCGTTTCCAAACGCAGTGATTCATTCGCTTCAATACCACACTTCTCT
GCTTGAGTCTCTTGATGCTAATCAGAACCAGGACGATTCGAGTATCGAGAGGTTTTGGGT
TCAACCATCGATAGAGAAGCTGTTGATGAAACGACACCGTTGGATTGAAAGATCTCCACC
GTGGAGAATCTTGTTTACACAATGTGGGTTTTCTCCGGCGAGTTTGAGTCAGATGGCGGA
AGCTCAGGCGGAGTGTTTGTTGCAGAGAAATCCGGTGAGAGGGTTTCACGTTGAGAAAAG
GCAGTCTTCACTTGTCATGTGTTGGCAAAGGAAAGAACTTGTTACTGTCTCTGCTTGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 73-K114085-0022-312-H09C-8409
GenBank Accession LN512946 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K114085-0022-312-H09C-8409
TACAGTTCTTTCCTTTGCCACACATGACAAGTGAAGACTGCCTTTTCTCACGTGAAACCC
TCTCACCGGATTTCTCTGCAACAAACACTCCGCCTGAGCTTCCGCCATCTGACTCAAACT
CGCCGGAGAAAACCCACATTGTGTAAACAAGATTCTCCACGGTGGAGATCTTTCAATCCA
ACGGTGTCGTTTCATCAACAGCTTCTCTATCGATGGTTGAACCCAAAACCTCTCGATACT
CGAATCGTCCTGGTTCTGATTAGCATCAAGAGACTCAAGCAGAGAAGTGTGGTATTGAAG
CGAATGAATCACTGCGTTTGGAAACGGCGCGTCGTTACGGTCACATCCTCTGTCTGAGCA
AACGACGATGTTTGGTGATAGTTGTTTAAGGAATCGGAAGGATTAACGNGAAGGTAACCG
NGAAGGCGACGGAGTTTANCTGGAAGAATTCACTGCGATTGGGCTTCTTTTTCCGAAATG
AACGTAGAGANNGAGTGGCCAATAANNGCTGGGTTTANGAAGAAAGCTCTNNACGCTTAG
TAACNNTCGATTTCGAAAGGGAATCNTTGNNACTTCTCCGGCGAATGGTTTTGAGAATTT
TCCTNNCTGTGAAACNGAAGCNNTCGAACTCGTCGGAAGACTNGGTAGAAGGGTGGAGGA
GCAAAGAACTGGGTTAATT

Last Updated on 06.12.2018 16:11