Sequences from confirmation amplicon


Confirmation sequences for line 313A06 and hit At2g23950 are shown.
Line ID 313A06
Locus At2g23950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10189290
Primers used for PCR EQ19 - 8474, show primer details
   
Sequence Name 37-K102876-0022-313-A06C-8409
GenBank Accession LN502575 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K102876-0022-313-A06C-8409
TTGAAATATCCTGTAATGACCTTCAAAAATCCAAAACACAAAAACAGAGTTTGAAGAAAC
AAACAAAGGAGATACTTTTGTTTAATGAGGAAACAAACAAAATAATCTTACACTTGTCGA
AGATTAGTGAGATTTCCAATAGACCCAGATAAAGTTCCTGAAAGAGACTGACTTGGAGCT
CCTCTGGAATTGGGAAAAAACAAAACAAAGATGAAGAATTTGTCACCATTAATGACTATG
AAGACAAAAGCAAAGAAAACTTACAAGCCAATTACGAGGTTGTCTGAAGAACAAGAGATC
ATAGTCCAGCTACAAGGATCAACAGAAAACTCATCCCAGTTTTTGAAAACACCATGTGGA
TCATGTAACTCGTTCTTTATGTTTATCAACGCCTCCACTGTAACAAAGAATCACTCTAAA
TCAATTATTGGGTACATAAAGTTTGAAACTTTCGAATACCCATAAGAAAAAAAAGAGTCT
TTTCTCGTTTTTTGTTGCTTTGCTTACCTTCAGGGTTTCTGGGTTCAGAAGAGAGAGA
Primers used for PCR KH52 - 8474, show primer details
   
Sequence Name 78-K118656-0022-313-A06C-KH52
GenBank Accession LN513751 [GenBank]
Sequencing primer gene specific primer, KH52: 5'-TAGGAACAGGACCTCTGAGATTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K118656-0022-313-A06C-KH52
GAGTCTTTAAGTAAAAAGTAAGAAAAAAATTCAAACTTTATATACTCACAAGAAAGAGAG
GTGAGGGATTTGAGACAGAGAAGCAGGAAAGGGCCCAGATAATGAGTTGTTGTTCAACCT
CCTAATCAAATCATTACACAAACGTACGAGGTTTAAGTTAATGAAGACGAATCTTTGATT
GATTAAAATCTGAAAGAGAGAGAGAGACTTACAGATATTGGAGATTACTCAGCTGGTTAA
CAGAACCGGGGATTTCACCGGAGAACCAGCGGGTAAATAATGAAGATTTTCTTCTTGTCA
TTGAGTCGTAAGAGACTCTGTATGAACTGTTCGCCAGTCTTTACGGCGAGTTCTGTTAGG
TCCTCTATTTGAATCTTTGACTCCATGGGAATTGGGGATCCTCTAGAGTCGACATGCAAG
CTTGGCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACT
TAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCAC
CGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCG
   
Sequence Name 52-K118655-0022-313-A06C-8409
GenBank Accession LN507965 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K118655-0022-313-A06C-8409
TTGAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGA
TCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGA
TTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCC
AAGCTTGCATGTCGACTCTAGAGGATCCCCAATTCCCATGGAGTCAAAGATTCAAATAGA
GGACCTAACAGAACTCGCCGTAAAGACTGGCGAACAGTTCATACAGAGTCTCTTACGACT
CAATGACAAGAAGAAAATCTTCATTATTTACCCGCTGGTTCTCCGGTGAAATCCCCGGTT
CTGTTAACCAGCTGAGTAATCTCCAATATCTGTAAGTCTCTCTCTCTCTTTCAGATTTTA
ATCAATCAAAGATTCGTCTTCATTAACTTAAACCTCGTACGTTTGTGTAATGATTTGATT
AGGAGGTTGAACAACAACTCATTATCTGGGCCCTTTCCTGCTTCTCTGTCTCAAATCCCT
CACCTCTCTTTCTTGTGAGTATATAAAGTTTGAATTTTTTTCTTACTTTTTAACTTAAAG
ACTCATGTTTTTTGATGTTTTGGTTTCAGAGACTTGTCTTATAACAATCTCAGAGGCGCT
GGTTCCT

Last Updated on 06.12.2018 16:11