Sequences from confirmation amplicon


Confirmation sequences for line 313F11 and hit At5g37080 are shown.
Line ID 313F11
Locus At5g37080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14661660
Primers used for PCR 51ac - 8474, show primer details
   
Sequence Name 14-K106147-0022-313-F11C-51ac
GenBank Accession LN492516 [GenBank]
Sequencing primer gene specific primer, 51ac: 5'-TGTTGATTTACTTCATGGGAGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K106147-0022-313-F11C-51ac
TTCTAATACTGTGTTTAGTTAAGTACCTATAATTGTGATTGTATTGTAAATCAGGTTGGG
CAATGGAGAATACTTGGTTCCATTTTTGCCATTGATACCGATTGGGGATGGTTCTATTTT
GGATGTCCTAAATGTAACAGAAAAACTGAGCTGGTTAAAGAATCAACCAGCACTGGTAAG
ATGGTCAAGACACCTATGAAACCTAAGTTCTGGTGTGACAAATGTCAAGAGAGCATCACT
AATGTGGAGGCAAGGTAATAATGTTTTCTATTACTCTTTTATCTTGTACAATATAACAAA
ATTGTTTCTATTGGTATACAGAAAATATAAAGGTTTATAAGTGTATATTAATTATATATG
TACAATACATTTTACTCCAGGTACAAGCTTCATGTTCGTGTAATGGACCAGACAGCTGAA
ATTAAACTCATGGTGTTTGAAAATAATGCAACCAAGCTTATTGGGAAATCTTCAGAAGAG
TTAATATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 30-K106148-0022-313-F11C-8409
GenBank Accession LN499727 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K106148-0022-313-F11C-8409
TTTCCATAAGCTTGGTTGCATTATTTTCAAACACCATGAGTTTAATTTCAGCTGTCTGGT
CCATTACACGAACATGAAGCTTGTACCTGGAGTAAAATGTATTGTACATATATAATTAAT
ATACACTTATAAACCTTTATATTTTCTGTATACCAATAGAAACAATTTTGTTATATTGTA
CAAGATAAAAGAGTAATAGAAAACATTATTACCTTGCCTCCACATTAGTGATGCTCTCTT
GACATTTGTCACACCAGAACTTAGGTTTCATAGGTGTCTTGACCATCTTACCAGTGCTGG
TTGATTCTTTAACCAGCTCAGTTTTTCTGTTACATTTAGGACATCCAAAATAGAACCATC
CCCAATCGGTATCAATGGCAAAAATGGAACCAAGTATTCTCCATTGCCCAACCTGATTTA
CAATACAATCACAATTATAGGTACTTAAACTAAACACAGTAATTTAGGAATTAGTGGAAA
TATACTTACGTCTCTCCCATGAATAANATCAAC

Last Updated on 06.12.2018 16:11