Sequences from confirmation amplicon


Confirmation sequences for line 314B04 and hit At4g34960 are shown.
Line ID 314B04
Locus At4g34960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16649138
Primers used for PCR 57cp - 8474, show primer details
   
Sequence Name 20-K108475-0022-314-B04C-57cp
GenBank Accession LN495245 [GenBank]
Sequencing primer gene specific primer, 57cp: 5'-ATATCAACATCGAGAAAAACCCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K108475-0022-314-B04C-57cp
TTGCTTCTCCTCATCCTAGTATCAAGAGAGGAAGTAAATCAGGCGCAAACATACACAAGG
CATATCACATTCTGCTGCTCCTCTATTAAACCTTCTCACTAACCAAGCAACCATAAATTC
AACAAAGAGAAACCTAACAATAATATGTCAGACCCGGAAAATAGAAAGCTAACCCAAGTT
CAACAATGTTATTCTGGTACAATAACCCAAAGTTCAAAACCATCATAAGAAGAATCTGAC
AAGCATTTGCCATGGAGCTTATAATGTGTGTGATACTCACTTAAACACTTTAATGTACTT
GGAGATAAAATGTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 04-K108474-0022-314-B04C-8409
GenBank Accession LN487952 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K108474-0022-314-B04C-8409
ATTTACATTGAACATTTTATCTCCAGTACATTAAAGTGTTTAAGTGAGTATCACACACAT
TATAAGCTCCATGGCAAATGCTTGTCAGATTCTTCTTATGATGGTTTTGAACTTTGGGTT
ATTGTACCAGAATAACATTGTTGAACTTGGGTTAGCTTTCTATTTTCCGGGTCTGACATA
TTATTGTTAGGTTTCTCTTTGTTGAATTTATGGTTGCTTGGTTAGTGAGAAGGTTTAATA
GAGGAGCAGCAGAATGTGATATGCCTTGTGTATGTTTGCGCCTGATTTACTTCCTCTCTT
GATACTTAGGATGAGGAGAAGCAAGTAATTGAAGATCATGAAATCACCAACAGGGTTTTT
CTCGATGTTGATA

Last Updated on 06.12.2018 16:11