Sequences from confirmation amplicon


Confirmation sequences for line 315G08 and hit At4g07950 are shown.
Line ID 315G08
Locus At4g07950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:4799050
Primers used for PCR 04aj - 8474, show primer details
   
Sequence Name 09-K106680-0022-315-G08C-8409
GenBank Accession LN490194 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K106680-0022-315-G08C-8409
TCGGACTGAGCCATTTACATTGAATATATCCTGTCCGGAAAATTCATGTCCATAAGAAAA
TTGGTTATTTTCTTAACCAGAAGTTGCTTCTTCTTTATCTCAACCCGTCTTTCGATATTG
GCAACGTATGGACATGTGGAACAGAAGAATCTCGAACTCCCACCTCCCTCGTAGCGCAAC
AGATTCCCACATGTGGGACAAAACTCCATCTTCCACCTCACCGATCAATTCGCTGCAAAA
TCATCCCCCACAAAAATCAATTCATAAAACTATGATATAGACAGATATATAGAGACAGAG
AGAGGGAAGGGCTGTTTTTAATACATTGTTCTACTGTGTTCGTGTAACAACCAGTTTTTA
CTTTAATAGCAAGGCAGGTCATGAAGTAACTTATAATTCAAGTAATCCATTCTTAACCGG
AAATAACCAATACTTTTCCATAAATTCAACTTTACATTCATTAGACTTTGGTTTAATAAT
GAAACCAACTCCAAAATCTAGCAATTTAATAATACCACAATTCAATCCACTGACTTAATA
ATTCGATCCACTAATCCAATCTATACTCACGAGTGACGACCACATTCTAAACCAACAATT
CAATTTTTATTGCCAAGATTGACCACATTTTCAATAGAATATACATATTTAATCAAGTGT
GGTGAAATCAACAAACCCAATAGCGAAGCTATTGTTCATACATAAACACAAGCACGAAAG
AG
   
Sequence Name 25-K106681-0022-315-G08C-04aj
GenBank Accession LN497461 [GenBank]
Sequencing primer gene specific primer, 04aj: 5'-TGTTACAGAAACTCTCTTTCGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K106681-0022-315-G08C-04aj
TGGGTTTGTTGATTTCACCACACTTGATTAAATATGTATATTCTATTGAAAATGTGGTCA
ATCTTGGCAATAAAAATTGAATTGTTGGTTTAGAATGTGGTCGTCACTCGTGAGTATAGA
TTGGATTAGTGGATCGAATTATTAAGTCAGTGGATTGAATTGTGGTATTATTAAATTGCT
AGATTTTGGAGTTGGTTTCATTATTAAACCAAAGTCTAATGAATGTAAAGTTGAATTTAT
GGAAAAGTATTGGTTATTTCCGGTTAAGAATGGATTACTTGAATTATAAGTTACTTCATG
ACCTGCCTTGCTATTAAAGTAAAAACTGGTTGTTACACGAACACAGTAGAACAATGTATT
AAAAACAGCCCTTCCCTCTCTCTGTCTCTATATATCTGTCTATATCATAGTTTTATGAAT
TGATTTTTGTGGGGGATGATTTTGCAGCGAATTGATCGGTGAGGTGGAAGATGGAGTTTT
GTCCCACATGTGGGAATCTGTTGCGCTACGAGGGAGGTGGGAGTTCGAGATTCTTCTGTT
CCACATGTCCATACGTTGCCAATATCGAAAGACGGGTTGAGATAAAGAAGAAGCAACTTC
TGGTTAAGAAAATAACCAATTTTCTTATGGACATGAATTTTCCGGACAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTC

Last Updated on 06.12.2018 16:11