Sequences from confirmation amplicon


Confirmation sequences for line 316H07 and hit At5g44300 are shown.
Line ID 316H07
Locus At5g44300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17845385
Primers used for PCR 16qz - 8474, show primer details
   
Sequence Name 20-K115950-0022-316-H07C-16qz
GenBank Accession LN495358 [GenBank]
Sequencing primer gene specific primer, 16qz: 5'-TTACCATGGTGAAATTTTGAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K115950-0022-316-H07C-16qz
ATCATTTAATATATACATTCCTCTAATCGGAGTGTTACATATGTGGGGGTTTAGAAGGAA
TGTCAGTGGCCGGATCGGTATCTCTTCCAGCGAGTCCGGCTACTCCGGTGACACCAGGAT
CTGGTCGGAAAGTGGATGTGTGGAGGAGTGTATTTCATCCGGCAAGTAATGTGACCACAA
GGGAGATTGGTGCTAATGTCTTTGACAAACCCTCTCATCCTAATTCTCCTACGGTTTATG
ACTGGTAAATGATTTATATTATCAATTTTATAGTTGCATCTCATAGATTTTAAGATCCAA
TTTAACCTTATAGGTATACATTTTTTATATGAATCTAACAATGGGTATTAAGGGTTACAA
AACTTGTAATTAAATTGCGATTAGTTTTTTTATTGATTACAAAAAGTGTAGTTTTGGTTG
TTGTTGTTGTTGCTGCATGACAGGATGTATAGCAACGAGACCAGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 10-K115949-0022-316-H07C-8409
GenBank Accession LN490807 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K115949-0022-316-H07C-8409
CATTTACATTGAATATATCCTGGTCTCGTTGCTATACATCCTGTCATGCAGCAACAACAA
CAACAACCAAAACTACACTTTTTGTAATCAATAAAAAAACTAATCGCAATTTAATTACAA
GTTTTGTAACCCTTAATACCCATTGTTAGATTCATATAAAAAATGTATACCTATAAGGTT
AAATTGGATCTTAAAATCTATGAGATGCAACTATAAAATTGATAATATAAATCATTTACC
AGTCATAAACCGTAGGAGAATTAGGATGAGAGGGTTTGTCAAAGACATTAGCACCAATCT
CCCTTGTGGTCACATTACTTGCCGGATGAAATACACTCCTCCACACATCCACTTTCCGAC
CAGATCCTGGTGTCACCGGAGTAGCCGGACTCGCTGGAAGAGATACCGATCCGGCCACTG
ACATTCCTTCTAAACCCCCACATATGTAACACTCCGATTAGAGGAATGTATATATTTAAA
TGATATGACAATTAGAACAATATGATATGATTTAATTATTGACTGTTTCAAAATTTCACC
ATGGTAAA

Last Updated on 06.12.2018 16:11