Sequences from confirmation amplicon


Confirmation sequences for line 318A06 and hit At1g77390 are shown.
Line ID 318A06
Locus At1g77390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29084228
Primers used for PCR AS07 - 8474, show primer details
   
Sequence Name 23-K018491-022-318-A06C-AS07
GenBank Accession LN496361 [GenBank]
Sequencing primer gene specific primer, AS07: 5'-TGAACAATTCACCTTCAGAGATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K018491-022-318-A06C-AS07
ATTTACAGATTCAATTCCAGGATAAACCCTAAAAACTACATTTCTCGACGAACAAACACA
GGAATTGAAAAAGGAACAAAAAGTAGAGAGAATCGAAGAGAAATTTTACCAGAGTAGACG
ACGGTGAAGGATTTCTAGATCCATTCTTCTGATTTGTGATGTCGCCGAGGGGAGCACGAC
GGTTTCCAACATCGCGGAGTGAGGTTCGAGTCTTGGCTAAGTTCGGACGAGGAATCGGAT
TCTCCTGAGATAGATTTCTCGACGAAGAAGACATTGTTGATGAAGCAATCGAAAATATCT
CAGGCTCGGCGTTCTCGTTTTCCGGTGAGATTTAGGGTTTCTCTTCTGCGAGAGTGAGAG
AGAGAAACCCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGA
AAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAA
ACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 24-K018491-022-318-A06C-8409
GenBank Accession LN496803 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K018491-022-318-A06C-8409
TGATCCATGTAGGGTTTCTCTCTCTCACTCTCGCAGAAGAGAAACCCTAAATCTCACCGG
AAAACGAGAACGCCGAGCCTGAGATATTTTCGATTGCTTCATCAACAATGTCTTCTTCGT
CGAGAAATCTATCTCAGGAGAATCCGATTCCTCGTCCGAACTTAGCCAAGACTCGAACCT
CACTCCGCGATGTTGGAAACCGTCGTGCTCCCCTCGGCGACATCACAAATCAGAAGAATG
GATCTAGAAATCCTTCACCGTCGTCTACTCTGGTAAAATTTCTCTTCGATTCTCTCTACT
TTTTGTTCCTTTTTCAATTCCTGTGTTTGTTCGTCGAGAAATGTAGTTTTTAGGGTTTAT
CCTGGAATTGAATCTGTAAATGTTTCAATCTCTGAAGGTGAATTGTTC

Last Updated on 06.12.2018 16:11