Genotyping and primer details


Genotyping and primer details for 318A06 - At1g77390
Line ID 318A06
Locus At1g77390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29084228 of line 318A06 Chr1:29084228
   
Insertion allele amplicon 8474 - AS07
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AS07: 5'-TGAACAATTCACCTTCAGAGATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29083836
   
wt amplicon (over InsPos) 07em - AS07
wt amplicon start Chr1:29083836
wt amplicon end Chr1:29084618
wt amplicon size 783 bp
Locus specific primer AS07: 5'-TGAACAATTCACCTTCAGAGATTG-3'
2nd locus specific primer 07em: 5'-CTAACAGTTTTTACCTGCAGGAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11