Sequences from confirmation amplicon


Confirmation sequences for line 318D10 and hit F14G6 are shown.
Line ID 318D10
Locus F14G6 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28733775
Primers used for PCR PH91 - 8474, show primer details
   
Sequence Name 52-K021336-022-318-D10C-8409
GenBank Accession LN507682 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K021336-022-318-D10C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGAATTCTCAACTTGAACATATATGATAGTC
AACACGACAATACGTTGTATTCCTTATATTGAAATGAAGCTAATTAAGGTCTATATGGAA
ACAAATATGTTATTTATTTATCAATGACGTATCACATTTTGACGAATAAAGAGATTAATG
TTATCCTTTACAAAATAATGAAATAAGAAAGAAACAGAAGTTTATGTTTGTCCAATTATC
CTCAATGTTTGTTGATATACGAATTTTGAGAATTCAAACTGAATACAAGAAATAAAAATG
TAGTGAGATAATAATACAAATTTGCATTATTTCTTCCAAGATTGTTTTCTTTAAGTAAAT
GAAAAGTATCTTGGATTATTACAGAAATGACCCTAAATCTTTTTGAATTTACAGTTTGTA
CCACCACCGAAT
   
Sequence Name 53-K021336-022-318-D10C-PH91
GenBank Accession LN508010 [GenBank]
Sequencing primer gene specific primer, PH91: 5'-TTTCTTATTTTATTCGGTGGTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K021336-022-318-D10C-PH91
AAACTGTAAATTCAAAAAGATTTAGGGTCATTTCTGTAATAATCCAAGATACTTTTCATT
TACTTAAAGAAAACAATCTTGGAAGAAATAATGCAAATTTGTATTATTATCTCACTACAT
TTTTATTTCTTGTATTCAGTTTGAATTCTCAAAATTCGTATATCAACAAACATTGAGGAT
AATTGGACAAACATAAACTTCTGTTTCTTTCTTATTTCATTATTTTGTAAAGGATAACAT
TAATCTCTTTATTCGTCAAAATGTGATACGTCATTGATAAATAAATAACATATTTGTTTC
CATATAGACCTTAATTAGCTTCATTTCAATATAAGGAATACAACGTATTGTCGTGTTGAC
TATCATATATGTTCAAGTTGAGAATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11