Sequences from confirmation amplicon


Confirmation sequences for line 318E10 and hit At2g47640 are shown.
Line ID 318E10
Locus At2g47640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19537528
Primers used for PCR BV23 - 8474, show primer details
   
Sequence Name 49-K106193-0022-318-E10C-BV23
GenBank Accession LN506849 [GenBank]
Sequencing primer gene specific primer, BV23: 5'-GTGTTGAATTCCTCTTCCTCAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K106193-0022-318-E10C-BV23
GATGATTAGAAACACATTTAAGGAGAGAACACATCCTTAAGAGTATCATACAAGCAGGAA
ACTAATGGAGTTTATAACAGCAACAATCAAATGACATTACAACAAAGGGCAGAAGTGATG
AAAACATCCAATTCTCTACTGCCCATTCAACGCAGAACGGAACAAAAATTCAGTGAAAAT
CTTTAGTGGCACAGTCCTACTCAAGTACTAGAGACAGCTAAGATTGATGAAACTGGAAAC
AGTAGATGAATTATGGCAAACAGCGAATCGAGAAAAGCAGTAGCGAGAGAAATTAGAAGG
AAGCTTACATTGGTAAATCTACATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCACGNTTTATT
   
Sequence Name 65-K106194-0022-318-E10C-8409
GenBank Accession LN511407 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K106194-0022-318-E10C-8409
ATTTCCATGTAAGCTTCCTTCTATTTCTCTCGCTACTGCTTTTCTCGATTCGCTGTTTGC
CATAATTCATCTACTGTTTCCAGTTTCATCAATCTTAGCTGTCTCTAGTACTTGAGTAGG
ACTGTGCCACTAAAGATTTTCACTGAATTTTTGTTCCGTTCTGCGTTGAATGGGCAGTAG
AGAATTGGATGTTTTCATCACTTCTGCCCTTTGTTGTAATGTCATTTGATTGTTGCTGTT
ATAAACTCCATTAGTTTCCTGCTTGTATGATACTCTTAAGGATGTGTTCTCTCCTTAAAT
GTTGTTTCTAATTCATCATGCTTGATACTGTCCTTAGCAGGGCAAGACTGAGGAAGAGAA
NTTCAACA

Last Updated on 06.12.2018 16:11