Genotyping and primer details


Genotyping and primer details for 318E10 - At2g47640
Line ID 318E10
Locus At2g47640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19537528 of line 318E10 Chr2:19537528
   
Insertion allele amplicon 8474 - BV23
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BV23: 5'-GTGTTGAATTCCTCTTCCTCAGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19537891
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11