Sequences from confirmation amplicon


Confirmation sequences for line 319B08 and hit At5g13110 are shown.
Line ID 319B08
Locus At5g13110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4160524
Primers used for PCR 04tz - 8474, show primer details
   
Sequence Name 17-K116987-0022-319-B08C-04tz
GenBank Accession LN494026 [GenBank]
Sequencing primer gene specific primer, 04tz: 5'-GTCAATGGATGGACTAGGGTTATC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K116987-0022-319-B08C-04tz
TCGGCTGCTTTACGAAATCCCTCAACAGTATTTGGAGGAAGATCAAATTTTTAGGTGAAA
GATAAATGCACTTATAGCTTTGTTTTGTTGCAATGATAATACTATTGGATGCTTGGCTGA
TATGATATTTTGTTGCAATGATGATACTATTGGTTGCTTGGCTGATATGATAATGAAAAT
GTGTTCTTACAGGATAGACCATTACCTAGGAAAGGAGCTAGTCGAGAACTTATCTGTTCT
TCGATTCTCAAACCTTATATTTGAACACATATAAATCCACTTACGTTCAATTGAATAAAT
CCACTTAACGTGAGTTTTCGTTCCACTGCGGCCAATTTACAATTCAATATTTAAACTATC
CAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCACGGTTTATTAT
   
Sequence Name 05-K116986-0022-319-B08C-8409
GenBank Accession LN488520 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K116986-0022-319-B08C-8409
GCATTTCATTGAATAATCCTGGATAGTTAAATATTGAATTGTAAATTGGCCGCAGTGGAA
CGAAAACTCACGTTAAGTGGATTTATTCAATTGAACGTAAGTGGATTTATATGTGTTCAA
ATATAAGGTTTGAGAATCGAAGAACAGATAAGTTCTCGACTAGCTCCTTTCCTAGGTAAT
GGTCTATCCTGTAAGAACACATTTTCATTATCATATCAGCCAAGCAACCAATAGTATCAT
CATTGCAACAAAATATCATATCAGCCAAGCATCCAATAGTATTATCATTGCAACAAAACA
AAGCTATAAGTGCATTTATCTTTCACCTAAAAATTTGATCTTCCTCCAAATACTGCTTGA
GGGATTTCGTTAAAGCAGCCGAGGTTTCAGAATCTCGACCAAAAGGTTTCTCGACGATAA
CCCTAGTCCATCCATTGAC

Last Updated on 06.12.2018 16:11