Sequences from confirmation amplicon


Confirmation sequences for line 319H06 and hit At2g28700 are shown.
Line ID 319H06
Locus At2g28700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12318101
Primers used for PCR MK06 - 8474, show primer details
   
Sequence Name 07-K021340-022-319-H06C-8409
GenBank Accession LN489067 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K021340-022-319-H06C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAATATTTCCCGGACATGGAGAATTCAAAAA
TGAGGAGGAACTCCCTTCAGCAATAAGAAGTGGTGTCATGCCTTCATCTACTACAGTTGT
TGCCATGTTTCCAGTTGTAGCGGTTGGTGCCATATCCATTAATGAAGAAGATTCTCCGAT
TTCAAAATTTGGATTGTTGAGGGTTACATTTTTATGATGATAAAGATTTCTAAGATAGTG
ATCAATAAACTTACATAAGTCAAGACGATCATTATCATTCATAACAAAATCTCCCATGTT
CCCACCAAGACACTTGAACATGACCTCCTTTAAGCAAGTCTCCTTATTTTCTACAATTAA
TTTCTTACTTTGCTTTTCGACTTTTGAGATGTATAGATTGAGAAATTCTTCGTGGTT
   
Sequence Name 08-K021340-022-319-H06C-MK06
GenBank Accession LN489534 [GenBank]
Sequencing primer gene specific primer, MK06: 5'-GAACCACGAAGAATTTCTCAATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K021340-022-319-H06C-MK06
CTCAAAAGTCGAAAAGCAAAGTAAGAAATTAATTGTAGAAAATAAGGAGACTTGCTTAAA
GGAGGTCATGTTCAAGTGTCTTGGTGGGAACATGGGAGATTTTGTTATGAATGATAATGA
TCGTCTTGACTTATGTAAGTTTATTGATCACTATCTTAGAAATCTTTATCATCATAAAAA
TGTAACCCTCAACAATCCAAATTTTGAAATCGGAGAATCTTCTTCATTAATGGATATGGC
ACCAACCGCTACAACTGGAAACATGGCAACAACTGTAGTAGATGAAGGCATGACACCACT
TCTTATTGCTGAAGGGAGTTCCTCCTCATTTTTGAATTCTCCATGTCCGGGAAATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
TTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
ACATT

Last Updated on 06.12.2018 16:11