Sequences from confirmation amplicon


Confirmation sequences for line 320A07 and hit At3g44870 are shown.
Line ID 320A07
Locus At3g44870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16382293
Primers used for PCR JD06 - 8474, show primer details
   
Sequence Name 13-K024844-022-320-A07C-8409
GenBank Accession LN491869 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K024844-022-320-A07C-8409
GTGAATGAAGTCGACATGTCTCTCTTTTAACTCCTGTCTTGTGATGATTTCTTTATATGT
GCGTGAGGATGTGATGAATGTTTTAGTTTCAAGCCTTTGTGCTTATTTATATCTATCAAT
CGGCTTGCCATTGAGGGTTTGAGTATTGCTAGTATTAATAAACATATTGGCCACGTTTTA
TTAAACAAATTTTCGAATACATTAACTAATAAACATAATTATCATATTTTAATACTATAA
GCCTATGCAGGGAATCATTTGACCATTCACTTTTCACGATGCAGCCCAATAATGTGGATT
TTGCAGCCTGTCAGGGATCACTTGACCACCGCAACAACCAAATTCATTATTAATTAGAAC
TTTTAGAAATGATTAAACATGTGTGCTAAATAGGATATAACTATTAATTTAAAATGTGTC
ATTAAAAACTTGATTGAT
   
Sequence Name 14-K024844-022-320-A07C-JD06
GenBank Accession LN492339 [GenBank]
Sequencing primer gene specific primer, JD06: 5'-TGTTTGCAAGAGAATCAATCAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K024844-022-320-A07C-JD06
ATGACACATTTTAAATTAATAGTTATATCCTATTTAGCACACATGTTTAATCATTTCTAA
AAGTTCTAATTAATAATGAATTTGGTTGTTGCGGTGGTCAAGTGATCCCTGACAGGCTGC
AAAATCCACATTATTGGGCTGCATCGTGAAAAGTGAATGGTCAAATGATTCCCTGCATAG
GCTTATAGTATTAAAATATGATAATTATGTTTATTAGTTAATGTATTCGAAAATTTGTTT
AATAAAACGTGGCCAATATGTTTATTAATACTAGCAATACTCAAACCCTCAATGGCAAGC
CGATTGATAGATATAAATAAGCACAAAGGCTTGAAACTAAAACATTCATCACATCCTCAC
GCACATATAAAGAAATCATCACAAGACAGGAGTTAAAAGAGAGACATGTCGACTTCATTC
ACACGTGGCAAAAACGTGTAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAA
ATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11