Sequences from confirmation amplicon


Confirmation sequences for line 320E07 and hit At1g48500 are shown.
Line ID 320E07
Locus At1g48500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17932373
Primers used for PCR 15vd - 8474, show primer details
   
Sequence Name 18-K117753-0022-320-E07C-15vd
GenBank Accession LN494491 [GenBank]
Sequencing primer gene specific primer, 15vd: 5'-GAGAATGACCAATCCATCATACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K117753-0022-320-E07C-15vd
ACTAGGAAACACTCAGATCTAGCTATGCTAAAGTGGACGACTCTTAACATTAGATTCATC
AACAATGAACAAATCTGCATAAAGCCAAACACACTGGGGAAAATAGTGATCATACAAATA
CTTTCAAGAAATGGCACGTTTCGGAATATAAAAAGACAAGAACTAACTATTAAATTCCTC
GTGGGTAAGTAAATTATTCATCCAAATTGTAACTTGATCTAAATTTAATACCCAACATGA
TCCTAACTTAATCAATGAGTCTACTTGGATAGAAATAAAACTTAATCAAGTCACAATCAA
TCTTGCTTTTATATAATTCAACTTGCATTTGTTGTTAAATCTCTAATCGTCAGCCGGTCG
CTATATAACGCATTCTTTTCCCAACATTTCACGTCTTCGTTTTTAAATAATACTGATAGA
ATTCAAGTTGCTTAATAAACCTTAAAAAATGGCATAAACTTTTCAAAAAGAATAACCAGC
CAATGTTCTAATTCTCAATATTCAATCCGGCTTTGAAAAAAACAATCTTTTCTAAAGAAG
ATACTATTGTATTGAAACCAATTGATCAATTTTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGT
   
Sequence Name 02-K117752-0022-320-E07C-8409
GenBank Accession LN487217 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K117752-0022-320-E07C-8409
CAAATTGATTCAATTGGTTTCATACAATAGTATCCTTCCTTTAGAAAAGATTGTTTTTTT
CAAAGCCGGATTGAATATTGAGAATTAGAACATCGGCTGGTTATTCTTTTTGAAAAGTTT
ATGCCATTTTTTAAGGTTTATTAAGCAACTTGAATTCTATCAGTATTATTTAAAAACGAA
GACGTGAAATGTTGGGAAAAGAATGCGTTATATAGCGACCGGCTGACGATTAGAGATTTA
ACAACAAATGCAAGTTGAATTATATAAAAGCAAGATTGATTGTGACTTGATTAAGTTTTA
TTTCTATCCAAGTAGACTCATTGATTAAGTTAGGATCATGTTGGGTATTAAATTTAGATC
AAGTTACAATTTGGATGAATAATTTACTTACCCACGAGGAATTTAATAGTTAGTTCTTGT
CTTTTTATATTCCGAAACGTGCCATTTCTTGAAAGTATTTGTATGATCACTATTTTCCCC
AGTGTGTTTGGCTTTATGCAGATTTGTTCATTGTTGATGAATCTAATGTTAAGAGTCGTC
CACTTTAGCATAGCTAGATCTGAGTGTTTCCTAGTTTGATAAAATCTAAAGACATTTGCT
CATGTTTCAGCCCCAAGTAGAGGTATGAT

Last Updated on 06.12.2018 16:11