Sequences from confirmation amplicon


Confirmation sequences for line 321A06 and hit At4g34480 are shown.
Line ID 321A06
Locus At4g34480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16481516
Primers used for PCR 06an - 8474, show primer details
   
Sequence Name 11-K106971-0022-321-A06C-8409
GenBank Accession LN491133 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K106971-0022-321-A06C-8409
TCACTTTTTATCTAGTTACTAGTTGAAACTACTATTGGTAGGGTGTGAAAGTAACGGGGA
GAGAAATCTTACTAGGGTTTTGGGAAGTGACTGTTGCGGTTTTAGAGAAATCGCAGTCTG
TAGGCTGCTTAGGAGATTTCTGAAAATACATATTCATGGCATATGCTGCGTGAGAGACCA
CATTGTTCGGCTCGAAACAAGCCCCTCCTGGTTGTATCGCACCACAGTCTATTCCATGTC
CGCACGCCCAATCCAAGCTCGCTTGTAACTCCTCATTCGTTGCACCCTTCTTTGGTACAC
ACCACCCCGACGATGTCACTTTTCCTGATGGTGTCTAATTTTATGATCAAAACATAACCT
CACAAATTAAAACATTGTTATTTATCTGCATTTGCGTTTACATGTTATAATAAATTACTT
TCCCTAAATAAAAACCACAACTTCTAGTATCTAACCTGACTACTGCTGCTACTCTTGGCA
AGGCCAACATCATAGACCATGGAAAGATCAGTTTTGAAAAGCCCGAAAGCCCGTTCTGAA
GATGGTCCAGGCTTTAGATTCTCATCATACAGTGCGAAA
   
Sequence Name 27-K106972-0022-321-A06C-06an
GenBank Accession LN498407 [GenBank]
Sequencing primer gene specific primer, 06an: 5'-TTTTCGCACTGTATGATGAGAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K106972-0022-321-A06C-06an
GGCTTCGGGCTTTTCAAACTGATCTTTCCTGGTCTATGATGTTGGCCTTGCCAAGAGTAG
CAGCAGTAGTCAGGTTAGATACTAGAAGTTGTGGTTTTTATTTAGGGAAAGTAATTTATT
ATAACATGTAAACGCAAATGCAGATAAATAACAATGTTTTAATTTGTGAGGTTATGTTTT
GATCATAAAATTAGACACCATCAGGAAAAGTGACATCGTCGGGGTGGTGTGTACCAAAGA
AGGGTGCAACGAATGAGGAGTTACAAGCGAGCTTGGATTGGGCGTGCGGACATGGAATAG
ACTGTGGTGCGATACAACCAGGAGGGGCTTGTTTCGAGCCGAACAATGTGGTCTCTCACG
CAGCATATGCCATGAATATGTATTTTCAGAAATCTCCTAAGCAGCCTACAGACTGCGATT
TCTCTAAAACCGCAACAGTCACTTCCCAAAACCCTAGTAAGATTTCTCTCCCCGTTACTT
TCACACCCTACCAATAGTAGTTTCAACTTAGTAACTTAGTATAAAAAGTTGTAATGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11