Sequences from confirmation amplicon


Confirmation sequences for line 321B08 and hit At5g61520 are shown.
Line ID 321B08
Locus At5g61520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24740843
Primers used for PCR BV26 - 8474, show primer details
   
Sequence Name 44-K105952-0022-321-B08C-BV26
GenBank Accession LN505190 [GenBank]
Sequencing primer gene specific primer, BV26: 5'-GGTAGCAGAAGAAGCAAGAAAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K105952-0022-321-B08C-BV26
CTGGCGGGAGATACTTATTTTGTGGTGGCTTCATGTGTCATGGCCGCCATGGGCGGTGTC
ATCTTCGGCTACGACATCGGGGTTTCAGGTTCACATATATATTCTTGCTAATTAGGATAT
ATATGTTCTTTCTTTTTTTTGTTTGTGTTTGTGTTTGCGTTTGCAAAAAGAATAAATCAT
ATTTTTGAAGTAGAAAACATTTTAGTTTTTCATAATATTTAAATGAAATGTTGTGGTTCT
CTAAACCTAAACATAAATGCAAACATGTAACTATATAAATATGTACATATGTAAATATTC
AATGACTTTCAATGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 60-K105953-0022-321-B08C-8409
GenBank Accession LN510264 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K105953-0022-321-B08C-8409
TTACAATTGAATATATCCTGCATTGAAAGTCATTGAATATTTACATATGTACATATTTAT
ATAGTTACATGTTTGCATTTATGTTTAGGTTTAGAGAACCACAACATTTCATTTAAATAT
TATGAAAAACTAAAATGTTTTCTACTTCAAAAATATGATTTATTCTTTTTGCAAACGCAA
ACACAAACACAAACAAAAAAAAGAAAGAACATATATATCCTAATTAGCAAGAATATATAT
GTGAACCTGAAACCCCGATGTCGTAGCCGAAGATGACACCGCCCATGGCGGCCATGACAC
ATGAAGCCACCACAAAATAAGTAATCTTCCCGCCAGATACTGATTTAGCCATGGCTTCTT
TTCTTGCTCTNCCTGCTA

Last Updated on 06.12.2018 16:11