Sequences from confirmation amplicon


Confirmation sequences for line 321C09 and hit At3g61150 are shown.
Line ID 321C09
Locus At3g61150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22631152
Primers used for PCR PH08 - 8474, show primer details
   
Sequence Name 43-K017463-022-321-C09C-PH08
GenBank Accession LN504669 [GenBank]
Sequencing primer gene specific primer, PH08: 5'-AAAGAGTCAAACCCAACTTTTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K017463-022-321-C09C-PH08
TGTGTAGGCTTTCCATGACTCCACATCTTCTTCTTTTCCCTTTTTATCCTTTTCTCTTCT
CTCTCTTTTATTTTTTCTTTTGAAGTAAATTAATTTCACGAAACAAATCAGAGAACCTTT
TATATCTTCCTTTTTCGTCGTTACTATCGTCCTTTACCTTTTTCATTTACTGTTCTCTCT
CTCTCTCTATCTCTATCTCTATCTTCTATTCATGGACATGCACCGAAGCGACAAAGCTTA
GGGCTTTTCTCTCATCCATGGCGGATTTCTTCTATCTTCATTAAATATTTCCCTCTTTAT
TAAAATCTCATGAATTTCAACGGTTTTCTCGACGACGGTGCTGGAGCCTCAAAGCTACTC
TCTGATGCTCCGTACAACAACCACTTCTCTTTCTCCGCCGTAGACACCATGCTCGGAAGC
GCCGCCATTGCTCCTTCTCAGTCTCTTCCTTTCTCTTCTTCAGGCCTCTCTCTCGGACTC
GTAAGCTCACTATCTCCTCTTTTAATCTTCGATTTCTTCTTCTTCTACTACTTATGATAA
TCTCTGTAAATTTGGCAGCAAACAAATGGAGAAATGAGTAGAAACGGAGAGATTATGGAG
TCAAACGTAAGTCGTAAGAGTAGTAGAGGAAGAGATGTAGANAGCAGATCTGAAAGTGAT
AACGCTGAAGCTG
   
Sequence Name 44-K017463-022-321-C09C-8409
GenBank Accession LN505027 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K017463-022-321-C09C-8409
CATGTAGATTTCCCGGAATGAAGCCATCTAAANTCGTCACCGGAGACAGCTTCAGCGTTA
TCACTTTCAGATCTGCTTTCTACATCTTCTCCTCTACTACTCTTACGACTTACGTTTGAC
TCCATAATCTCTCCGTTTCTACTCATTTCTCCATTTGTTTGCTGCCAAATTTACAGAGAT
TATCATAAGTAGTAGAAGAAGAAGAAATCGAAGATTAAAAGAGGAGATAGTGAGCTTACG
AGTCCGAGAGAGAGGCCTGAAGAAGAGAAAGGAAGAGACTGAGAAGGAGCAATGGCGGCG
CTTCCGAGCATGGTGTCTACGGCGGAGAAAGAGAAGTGGTTGTTGTACGGAGCATCAGAG
AGTAGCTTTGAGGCTCCAGCACCGTCGTCGAGAAAACCGTTGAAATTCATGAGATTTTAA
TAAAGAGGGAAATATTTAATGAAGATAGAAGAAATCCGCCATGGATGAGAGAAAAGCCCT
AAGCTTTGTCGCTTCGGTGCATGTCCATGAATAGAAGATAGAGATAGAGATAGAGAGAGA
GAGAGAACAGTAAATGAAAAAGGTAAAGGACGATAGTAACGACGAAAAAGGAAGATATAA
AAGGTTCTCTGATTTGTTTCGTGAAATTAATTT

Last Updated on 06.12.2018 16:11