Sequences from confirmation amplicon


Confirmation sequences for line 321F04 and hit At5g44582 are shown.
Line ID 321F04
Locus At5g44582 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17981080
Primers used for PCR 10xp - 8474, show primer details
   
Sequence Name 35-K119542-0022-321-F04C-10xp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 10xp: 5'-AAAAGTAAATTAATCAATCGCCTACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K119542-0022-321-F04C-10xp
GCAACTTATAGAGAGTAACATCTAATTTCAACACGGCGACATATTATTTAGATGAATAAC
TACTTTGTAGAAAAGTGTTAAAAAGAAAGTTGATAAAAATTGTAAAATAAGAAAAGAGGA
GGGACATACCATTACCACCGCCACCACGGTCACTGGTTGATGATGGTGTTCCTATGTCAG
TATTAGGCTTATAATAACTCATCATCCTCCTTCCTATATATATACATGTGAAAGCAAAAC
TAGTAGTAAACCACAATAATGTCCAAGATGTAGTAAATGTAAATGGCTTCAATATTTTCC
ACAAAAATATTTTCCATTTCTCTCTCTCTTGGTTTAATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 03-K119541-0022-321-F04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K119541-0022-321-F04C-8409
ATTTACAATTGAATATATTAAACCAAGAGAGAGAGAAATGGAAAATATTTTTGTGGAAAA
TATTGAAGCCATTTACATTTACTACATCTTGGACATTATTGTGGTTTACTACTAGTTTTG
CTTTCACATGTATATATATAGGAAGGAGGATGATGAGTTATTTAAAGCCTAATACTGACA
TAGGAACACCATCATCAACCAGTGACCGTGGTGGCGGTGGTAATGGTATGTCCCTCCTCT
TTTCTTATTTTACAATTTTTATCAACTTTCTTTTTAACACTTTTCTACAAAGTACTTATT
CATCTAAATAATATGTCGCCGTGTTGAAATTAGATGTTACTCTCTATTAATGTTTGCTGC
TTTTTTTATTAGGTAGGCGATTGATTAATTAACTTTTAAACCCGGC

Last Updated on 06.12.2018 16:11