Sequences from confirmation amplicon


Confirmation sequences for line 321G09 and hit At1g62190 are shown.
Line ID 321G09
Locus At1g62190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22981715
Primers used for PCR CL83 - 8474, show primer details
   
Sequence Name 54-K032927-022-321-G09C-8409
GenBank Accession LN508384 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K032927-022-321-G09C-8409
TTACAATTGAATAATCCTGATGTCCATGGAACAAAGTTTTGGACGAAAGTAACTTATTTA
AGGCATTGGAGATGGCGTTGTTTTTCCAGTTCGGTGTGAGACCAAACTCGTGGAACGAGC
CAAACTCTGACTGGACAGAAGAAACCGAGACTAATTTCTTTACAAAGATTTAAAGAATAT
CTGAATATGTATGAGTGTGTCGAATAAAGAGCATGTATTAGGTTTAAGTATCAGATCTTG
TAAGCTGATTAAACCTCCATTGTTGATGATCTTTCGTAAGCATGCAAACTTCAATAACCT
GAACTACTACTTTTTACAAATAACCTTTCTAGTCTCTTTTTACATGGCGAAAACATTA
   
Sequence Name 54-K032927-022-321-G09C-CL83
GenBank Accession LN508385 [GenBank]
Sequencing primer gene specific primer, CL83: 5'-ATAATGTTTCCGCCATGTAAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K032927-022-321-G09C-CL83
TAGTTCAGGTTATTGAAGTTTGCATGCTTACGAAAGATCATCAACAATGGAGGTTTAATC
AGCTTACAAGATCTGATACTTAAACCTAATACATGCTCTTTATTCGACACACTCATACAT
ATTCAGATATTCTTTAAATCTTTGTAAAGAAATTAGTCTCGGTTTCTTCTGTCCAGTCAG
AGTTTGGCTCGTTCCACGAGTTTGGTCTCACACCGAACTGGAAAAACAACGCCATCTCCA
ATGCCTTAAATAAGTTACTTTCGTCCAAAACTTTGTTCCATGGACATCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
TTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
ACATTC

Last Updated on 06.12.2018 16:11